Gastrokine 1 Antibody (759821)

Western blot shows lysates of mouse stomach tissue and rat stomach tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Gastrokine 1 Monoclonal Antibody (Catalog # MAB7287) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Gastrokine 1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Gastrokine 1 was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Gastrokine 1 Monoclonal Antibody (Catalog # MAB7287) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to gastric glands. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clone: 759821
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Gastrokine 1 Antibody (759821) Summary

• Immunogen: E. coli-derived recombinant mouse Gastrokine 1
  Tyr38-Tyr201
  Accession # NP_079742
• Specificity: Detects mouse Gastrokine 1 in direct ELISAs and mouse and rat Gastrokine 1 in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) Gastrokine 1 and no cross-reactivity with rhBlottin is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: GKN1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Gastrokine 1 Antibody (759821)

• 18 kDa antrum mucosa protein
• AMP18
• AMP-18
• AMP18BRICHOS domain containing 1
• BRICD1
• CA11
• CA11MGC70354
• FOV
• Foveolin
• Gastrokine 1
• gastrokine-1
• GKN1
• Protein CA11

Background

Gastrokine 1 (GKN1; also CA11 and AMP-18) is a 17-20 kDa member of the Gastrokine protein family. It has limited expression, being restricted to mucous secreting pyloric antrum epithelial cells. Gastrokine 1 appears to promote epithelial cell proliferation and migration, and induce the formation of tight junctions between epithelial cells. By contrast, gastrokine 1 induces Fas expression in tumor cells, resulting in apoptosis. Mature mouse gastrokine 1 is 166 amino acids (aa) in length (aa 36-201 of SwissProt accession Q9CR36 or aa 19-184 of accession NP_079742). Based on the SwissProt sequence, it possesses one BRICHOS domain (aa 71-165) that contains a mitogenic sequence (aa 113-131). There is one potential alternative start site at Met18. Over aa 38-201 (accession Q9CR36), mouse gastrokine 1 shares 65% and 92% aa sequence identity with human and rat gastrokine 1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Gastrokine 1 Antibody (MAB7287)(1)

Publication using MAB7287

• AC Overstreet, BE Grayson, A Boger, D Bakke, EM Carmody, CE Bales, SC Paski, SF Murphy, CR Dethlefs, KJ Shannon, KR Adlaka, CE Wolford, VJ Campiti, CV Raghunanda, RJ Seeley, DL Boone Gastrokine-1, an anti-amyloidogenic protein secreted by the stomach, regulates diet-induced obesity Scientific Reports, 2021-05-04;11(1):9477. 2021-05-04 [PMID: 33947892] (IHC, Mouse)

Bioinformatics

• Gene Symbol: GKN1
• Uniprot:
  • NP_079742()