Gastrokine 1 Antibody (759821) [Alexa Fluor® 647]

• Reactivity: Mu
• Applications: WB, IHC
• Clone: 759821
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 647

Gastrokine 1 Antibody (759821) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant mouse Gastrokine 1
  Tyr38-Tyr201
  Accession # NP_079742
• Specificity: Detects mouse Gastrokine 1 in direct ELISAs and mouse and rat Gastrokine 1 in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) Gastrokine 1 and no cross-reactivity with rhBlottin is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Gastrokine 1 Antibody (759821) [Alexa Fluor® 647]

• 18 kDa antrum mucosa protein
• AMP18
• AMP-18
• AMP18BRICHOS domain containing 1
• BRICD1
• CA11
• CA11MGC70354
• FOV
• Foveolin
• Gastrokine 1
• gastrokine-1
• GKN1
• Protein CA11

Background

Gastrokine 1 (GKN1; also CA11 and AMP-18) is a 17-20 kDa member of the Gastrokine protein family. It has limited expression, being restricted to mucous secreting pyloric antrum epithelial cells. Gastrokine 1 appears to promote epithelial cell proliferation and migration, and induce the formation of tight junctions between epithelial cells. By contrast, gastrokine 1 induces Fas expression in tumor cells, resulting in apoptosis. Mature mouse gastrokine 1 is 166 amino acids (aa) in length (aa 36-201 of SwissProt accession Q9CR36 or aa 19-184 of accession NP_079742). Based on the SwissProt sequence, it possesses one BRICHOS domain (aa 71-165) that contains a mitogenic sequence (aa 113-131). There is one potential alternative start site at Met18. Over aa 38-201 (accession Q9CR36), mouse gastrokine 1 shares 65% and 92% aa sequence identity with human and rat gastrokine 1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.