Gastrokine 1 Antibody (759821) [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: WB, IHC
• Clone: 759821
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 594

Gastrokine 1 Antibody (759821) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant mouse Gastrokine 1
  Tyr38-Tyr201
  Accession # NP_079742
• Specificity: Detects mouse Gastrokine 1 in direct ELISAs and mouse and rat Gastrokine 1 in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) Gastrokine 1 and no cross-reactivity with rhBlottin is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Gastrokine 1 Antibody (759821) [Alexa Fluor® 594]

• 18 kDa antrum mucosa protein
• AMP18
• AMP-18
• AMP18BRICHOS domain containing 1
• BRICD1
• CA11
• CA11MGC70354
• FOV
• Foveolin
• Gastrokine 1
• gastrokine-1
• GKN1
• Protein CA11

Background

Gastrokine 1 (GKN1; also CA11 and AMP-18) is a 17-20 kDa member of the Gastrokine protein family. It has limited expression, being restricted to mucous secreting pyloric antrum epithelial cells. Gastrokine 1 appears to promote epithelial cell proliferation and migration, and induce the formation of tight junctions between epithelial cells. By contrast, gastrokine 1 induces Fas expression in tumor cells, resulting in apoptosis. Mature mouse gastrokine 1 is 166 amino acids (aa) in length (aa 36-201 of SwissProt accession Q9CR36 or aa 19-184 of accession NP_079742). Based on the SwissProt sequence, it possesses one BRICHOS domain (aa 71-165) that contains a mitogenic sequence (aa 113-131). There is one potential alternative start site at Met18. Over aa 38-201 (accession Q9CR36), mouse gastrokine 1 shares 65% and 92% aa sequence identity with human and rat gastrokine 1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • NP_079742()