Gasdermin like Antibody (6F4K3) - Cleaved, C-terminal Fragment

Western Blot: Gasdermin like Antibody (6F4K3) - Cleaved, C-terminal Fragment [NBP3-33477] - Western blot analysis of lysates from GSDMB-C-6-his protein, using Gasdermin like Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 0.3s.WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Western Blot: Gasdermin like Antibody (6F4K3) - Cleaved, C-terminal Fragment [NBP3-33477] - Western Blot analysis of lysates from 293T GSDMB stable cells and 293T GSDMB stable cells transfected with GZMA using Gasdermin like Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 6F4K3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Gasdermin like Antibody (6F4K3) - Cleaved, C-terminal Fragment Summary

• Description: Novus Biologicals Rabbit Gasdermin like Antibody (6F4K3) - Cleaved, C-terminal Fragment (NBP3-33477) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to residues surrounding Ser245 of human Gasdermin like (NP_001159430.1).
  
  Sequence:
  GKSLG
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GSDMB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for Gasdermin like Antibody (6F4K3) - Cleaved, C-terminal Fragment

• gasdermin B
• gasdermin-B
• Gasdermin-like protein
• gasdermin-like
• GSDMLPRO2521

Background

The Gasdermin like gene encodes a member of the gasdermin-domain containing protein family. Other gasdermin-family genes are implicated in the regulation of apoptosis in epithelial cells, and are linked to cancer. Multiple transcript variants encoding different isoform

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSDMB