Aiolos/IKZF3 Antibody - BSA Free

Western Blot: Aiolos/IKZF3 Antibody [NBP2-24495] - Total protein from Jurkat cells and human tonsil was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-IkZF3 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: Aiolos/IKZF3 Antibody [NBP2-24495] - IKZF3 antibody was tested in Jurkat cells at a 1:40 dilution against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue), respectively.

Western Blot: Aiolos/IKZF3 Antibody [NBP2-24495] - Analysis of IKZF3 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 0.25 ug/mL.

• Reactivity: Hu, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Aiolos/IKZF3 Antibody - BSA Free Summary

• Immunogen: A portion of amino acids 350-400 of human IKZF3 was used as the immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IKZF3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry
  • Western Blot 0.25 - 1.0 ug/mL
• Application Notes: Use in IHC reported in scientific literature (PMID:34207079)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: canine (88%), mouse (88%), rat (88%), equine (82%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Aiolos/IKZF3 Antibody - BSA Free

• AIO
• Aiolos
• IKAROS family zinc finger 3 (Aiolos)
• Ikaros family zinc finger protein 3
• IKZF3
• zinc finger protein Aiolos
• ZNFN1A3
• ZNFN1A3subfamily 1A, 3 (Aiolos)

Background

IKZF3 encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. At least six alternative transcripts encoding different isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aiolos/IKZF3 Antibody (NBP2-24495)(13)

Publications using NBP2-24495

• Zou Y, Liu B, Li L Et al. IKZF3 deficiency potentiates chimeric antigen receptor T cells targeting solid tumors Cancer letters 2021-10-20 [PMID: 34687790] (WB)
• Chen H, Liu J, Kaniskan HU Et al. Folate-Guided Protein Degradation by Immunomodulatory Imide Drug-Based Molecular Glues and Proteolysis Targeting Chimeras Journal of medicinal chemistry 2021-08-26 [PMID: 34378936]
• Barankiewicz J, Szumera-Cieckiewicz A, Salomon-Perzynski A et al. The CRBN, CUL4A and DDB1 Expression Predicts the Response to Immunomodulatory Drugs and Survival of Multiple Myeloma Patients Journal of Clinical Medicine 2021-06-18 [PMID: 34207079] (IHC, Human)
• Walker, Z J, Idler, B Et al. Exploiting Protein Translation Dependence in Multiple Myeloma with Omacetaxine-Based Therapy. Clin Cancer Res 2021-02-01 [PMID: 33109736] (ICC/IF, Human)
• Wang Z, Zhou G, Risu N et al. Lenalidomide Enhances CAR-T Cell Activity Against Solid Tumor Cells Cell Transplant 2020-09-24 [PMID: 32967454] (WB, Human)
• Liu J, Chen H, Ma L et al. Light-induced control of protein destruction by opto-PROTAC Sci Adv 2020-02-01 [PMID: 32128407] (WB, Human)
• Kim E, Ten Hacken E, Sivina M, et al. The BET inhibitor GS-5829 targets chronic lymphocytic leukemia cells and their supportive microenvironment Leukemia 2019-12-20 [PMID: 31862959]
• Zhu YX, Shi CX, Bruins LA et al. Identification of lenalidomide resistance pathways in myeloma and targeted resensitization using cereblon replacement, inhibition of STAT3 or targeting of IRF4 Blood Cancer J 2019-02-11 [PMID: 30741931] (WB, Human)
• Costa F, Vescovini R, Bolzoni M et al. Lenalidomide increases human dendritic cell maturation in multiple myeloma patients targeting monocyte differentiation and modulating mesenchymal stromal cell inhibitory properties Oncotarget 2017-08-08 [PMID: 28881793] (WB, Human)
• Uehara T, Minoshima Y, Sagane K et al. Selective degradation of splicing factor CAPERalpha by anticancer sulfonamides. Nat. Chem. Biol. 2017-04-24 [PMID: 28437394]
• Nguyen TV, Li J, Lu CJ et al. p97/VCP promotes degradation of CRBN substrate glutamine synthetase and neosubstrates. Proc. Natl. Acad. Sci. U.S.A. 2017-03-20 [PMID: 28320958] (WB, Human)
• Gao S, Geng C, Song T et al. Activation of c-Abl kinase potentiates the anti-myeloma drug lenalidomide by promoting DDA1 recruitment to the CRL4 ubiquitin ligase J. Biol. Chem 2017-01-13 [PMID: 28087699]
• Zhu YX, Braggio E, Shi CX et al. Identification of cereblon binding proteins and relationship with response and survival following pomalidomide and dexamethasone in multiple myeloma. Blood. 2014-06-09 [PMID: 24914135] (WB, Human)
  
  Details:
  Figs 3 & 5: IKZF1, IKZF3 or cerebron (CRBN), lentiviral infected human myeloma cell lines (HMCLs) and infected HMCLs treated with IKZF1, IKZF3 or CRBN ShRNAs, respecitvely. The specificity of the IKZF3 pAb was validated in IKZF3-infected HMCLs and in IKZF3-shRNA infected cells by WB (Figs 3 & 5). The IKZF3 pAb recognized IKZF3 in WB that had been co-IP'd using a CRBN antibody, thereby helping to demonstrate that IKZF3 is a CRNB binding protein (Fig 3D).

Bioinformatics

• Gene Symbol: IKZF3
• Uniprot:
  • Q9UKT9()