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Aiolos/IKZF3 Antibody

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Western Blot: Aiolos/IKZF3 Antibody [NBP2-24495] - Total protein from Jurkat cells and human tonsil was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-IkZF3 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Western Blot: Aiolos/IKZF3 Antibody [NBP2-24495] - Total protein from Jurkat cells and human tonsil was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Immunocytochemistry/ Immunofluorescence: Aiolos/IKZF3 Antibody [NBP2-24495] - IKZF3 antibody was tested in Jurkat cells at a 1:40 dilution against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained ...read more
Western Blot: Aiolos/IKZF3 Antibody [NBP2-24495] - Analysis of IKZF3 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 0.25 ug/mL.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Aiolos/IKZF3 Antibody Summary

Immunogen
A portion of amino acids 350-400 of human IKZF3 was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IKZF3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.25 - 1.0 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:100
Publications
Read Publications using
NBP2-24495 in the following applications:

  • WB
    6 publications

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: canine (88%), mouse (88%), rat (88%), equine (82%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aiolos/IKZF3 Antibody

  • AIO
  • Aiolos
  • IKAROS family zinc finger 3 (Aiolos)
  • Ikaros family zinc finger protein 3
  • IKZF3
  • zinc finger protein Aiolos
  • ZNFN1A3
  • ZNFN1A3subfamily 1A, 3 (Aiolos)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Ikaros Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4984) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Bands were detected for Ikaros (1-8 spice forms) at approximately 37 to 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Ikaros/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human Ikaros Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4984) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The VPAC promoter was detected by standard PCR.
Simple Western lane view shows lysates of Nalm‑6 human Pre-B acute lymphocytic leukemia cell line, loaded at 0.2 mg/mL. Specific bands were detected for Ikaros at approximately 63-77 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Ikaros Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4984) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
(2 Publications)
Species: Hu
Applications: WB, Simple Western, ChIP, CyTOF-ready, ICC, ICFlow
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Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 µg of cytoplasmic (Cyto) and 15 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Helios Monoclonal Antibody (Catalog # MAB73091) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Helios at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Helios was detected in immersion fixed paraffin-embedded sections of human thymus using Mouse Anti-Human Helios Monoclonal Antibody (Catalog # MAB73091) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cells around Hassall's corpuscles. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Species: Hu
Applications: WB, IHC
IL-2 [Unconjugated]
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1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
(22 Reviews)
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Western Blot: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Analysis of Raji cell lysate using BOB1 Mouse Monoclonal Antibody (BOB1/2424).
Immunocytochemistry/Immunofluorescence: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Immunofluorescent staining of paraformaldehyde-fixed Raji cells using BOB1 Antibody (BOB1/2424).followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with Red Dot (red).
Immunohistochemistry-Paraffin: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Formalin-fixed, paraffin-embedded human Spleen stained with BOB1 Mouse Monoclonal Antibody (BOB1/2424).
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PA
BCR Antibody
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human BCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5129) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BCR at approximately 155 - 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Publication)
Species: Hu
Applications: WB
EBF-1 Antibody
(1G8)
H00001879-M02
Western Blot: EBF-1 Antibody (1G8) [H00001879-M02] - Analysis of EBF1 expression in transfected 293T cell line by EBF1 monoclonal antibody (M02), clone 1G8.Lane 1: EBF1 transfected lysate(64 KDa).Lane 2: Non-transfected lysate.
Western Blot: EBF-1 Antibody (1G8) [H00001879-M02] - Analysis of EBF1 over-expressed 293 cell line, cotransfected with EBF1 Validated Chimera RNAi ( Cat # H00001879-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EBF1 monoclonal antibody (M02), clone 1G8 (Cat # H00001879-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: EBF-1 Antibody (1G8) [H00001879-M02] - Detection limit for recombinant GST tagged EBF1 is approximately 0.1ng/ml as a capture antibody.
(2 Publications)
Species: Hu, Mu
Applications: WB, ChIP, ELISA, IP, RNAi, S-ELISA
Bcl-xL Antibody
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Western Blot: Bcl-xL Antibody [NB100-56104] - WB analysis of tissue lysates from breast surgical tumor tissue biopsies (Lanes 1-7). Bcl-XS is not detected and variable amounts of Bcl-XL (~30 kDa) is detected. Lane 8. Recombinant Bcl-XL (positive control).
Western Blot: Bcl-xL Antibody [NB100-56104] - Analysis of Bcl-xL in Daoy whole cell lysate using anti-Bcl-xL antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Bcl-xL Antibody [NB100-56104] - Formalin-fixed paraffin-embedded sections of human epidermis stained for Bcl-xL expression. The bottom image was negative for Bcl-xL expression. The adjacent stratum spinosum and middle stratum granulosum layers were both very positive for Bcl-xL expression. The top stratum corneum consisting mainly of dead or peeling cells were both negative for Bcl-xL expression.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC, IHC-Fr, IHC-P, IP
Gasdermin like
Antibody
NBP2-33648
Immunohistochemistry-Paraffin: Gasdermin like Antibody [NBP2-33648] - Staining of human Fallopian tube shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Gasdermin like Antibody [NBP2-33648] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Gasdermin like Antibody [NBP2-33648] - Staining of human pancreas shows low expression as expected.
Species: Hu
Applications: IHC, IHC-P
ZPBP2 Antibody
NBP1-82689
Western Blot: ZPBP2 Antibody [NBP1-82689] - Analysis in control (vector only transfected HEK293T lysate) and ZPBP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: ZPBP2 Antibody [NBP1-82689] - Staining of human lymph node.
Immunohistochemistry-Paraffin: ZPBP2 Antibody [NBP1-82689] - Staining of human endometrium shows low expression as expected.
Species: Hu
Applications: WB, IHC, IHC-P
Pax5/BSAP Antibody
NBP2-38790
Immunohistochemistry-Paraffin: Pax5/BSAP Antibody [NBP2-38790] - Staining of human duodenum shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Pax5/BSAP Antibody [NBP2-38790] - Paraffin-embedded alcohol fixed rat spleen tissue (40x). Antigen retrieval pH9. PAX5 dilution 1:500 overnight at 4C. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Pax5/BSAP Antibody [NBP2-38790] - Analysis in human lymph node and cerebral cortex tissues. Corresponding PAX5 RNA-seq data are presented for the same tissues.
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(1 Publication)
Species: Hu, Rt
Applications: IHC, IHC-P
ZNFN1A4 Antibody
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Western Blot: ZNFN1A4 Antibody [NBP2-55305] - Analysis in human cell line HDLM-2.
Immunocytochemistry/Immunofluorescence: ZNFN1A4 Antibody [NBP2-55305] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies. Antibody staining is shown in green.
Species: Hu
Applications: WB, ICC/IF
EGFR Antibody
(DH8.3) - EGFRvIII, M ...
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Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
(5 Publications)
Species: Hu
Applications: WB, ELISA, Flow, IHC, IP, CyTOF-ready
TCF-3/E2A Antibody
(826927)
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Western blot shows lysates of D3 mouse embryonic stem cell line and P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse TCF‑3/E2A Monoclonal Antibody (Catalog # MAB7650) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TCF‑3/E2A at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TCF‑3/E2A was detected in immersion fixed frozen sections of E9.5 mouse embryo using Rat Anti-Mouse TCF‑3/E2A Monoclonal Antibody (Catalog # MAB7650) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to branchial arch nuclei. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
(2 Publications)
Species: Mu
Applications: WB, IHC
AHR Antibody
NB100-128
Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-128] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti AHR [NB100-128] at a 1:100 dilution overnight at 4C and detected with an anti-goat DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: AHR Antibody [NB100-128] - Analysis of FFPE human liver using 1:200 conc. of AHR antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.
Gel Super Shift Assays: AHR Antibody [NB100-128] - EMSA validation of AHR antibody on U937 cells nuclear protein. Image submitted by verified customer - see review for protocol details.
(1 Review)
(12 Publications)
Species: Hu, Mu, Rt
Applications: GS, ICC/IF, IHC, IHC-P
Recombinant Human
PRSS33 GST (N-Term) P ...
H00260429-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
CD4 Antibody
NBP1-19371
Immunohistochemistry: CD4 Antibody [NBP1-19371] - Increased CD3+ and CD4+ T-cell occurrence in the brainstem of SHR-72 transgenic rat model for tauopathies. (A-D) Immunofluorescence staining showed CD4+ T-cells in SHR-72 transgenic animals. (E- H) Immunofluorescence staining showed more perivascular than brain parenchyma infiltrating CD4+ T-cells in SHR-72 transgenic animals. PLoS One. 2019 May 23;14(5):e0217216. doi: 10.1371/journal.pone.0217216.
Immunohistochemistry: CD4 Antibody [NBP1-19371] - DBZ inhibits the accumulation of CD4+ T cells and Th2 differentiation in the AAAs. (B) The representation of immunohistochemical staining for CD4+ in abdominal aorta from four groups (left). Bar graphs show the percentage of CD4+ positive cell areas (right; n=3 per group). Bar: 50 um.
Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.
(9 Reviews)
(29 Publications)
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
IL-4 [Unconjugated]
6507-IL/CF
IL-4 [Unconjugated]
(3 Publications)
Species: Hu
IRF8 Antibody
AF5117
Western blot shows lysates of human liver and kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human IRF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5117) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRF8 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
IRF8 was detected in immersion fixed paraffin-embedded sections of human lymphoma using 5 µg/mL Sheep Anti-Human IRF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5117) overnight at4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(1 Publication)
Species: Hu
Applications: WB, IHC
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Publications for Aiolos/IKZF3 Antibody (NBP2-24495)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(6)
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Human
(6)
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Showing Publications 1 - 9 of 9.
Publications using NBP2-24495 Applications Species
Wang Z, Zhou G, Risu N et al. Lenalidomide Enhances CAR-T Cell Activity Against Solid Tumor Cells Cell Transplant Sep 24 2020 [PMID: 32967454] (WB, Human) WB Human
Liu J, Chen H, Ma L et al. Light-induced control of protein destruction by opto-PROTAC Sci Adv Feb 1 2020 [PMID: 32128407] (WB, Human) WB Human
Kim E, Ten Hacken E, Sivina M, et al. The BET inhibitor GS-5829 targets chronic lymphocytic leukemia cells and their supportive microenvironment Leukemia Dec 20 2019 [PMID: 31862959]
Zhu YX, Shi CX, Bruins LA et al. Identification of lenalidomide resistance pathways in myeloma and targeted resensitization using cereblon replacement, inhibition of STAT3 or targeting of IRF4 Blood Cancer J Feb 11 2019 [PMID: 30741931] (WB, Human) WB Human
Costa F, Vescovini R, Bolzoni M et al. Lenalidomide increases human dendritic cell maturation in multiple myeloma patients targeting monocyte differentiation and modulating mesenchymal stromal cell inhibitory properties Oncotarget 2017 Aug 08 [PMID: 28881793] (WB, Human) WB Human
Uehara T, Minoshima Y, Sagane K et al. Selective degradation of splicing factor CAPERalpha by anticancer sulfonamides. Nat. Chem. Biol. Apr 24 2017 [PMID: 28437394]
Nguyen TV, Li J, Lu CJ et al. p97/VCP promotes degradation of CRBN substrate glutamine synthetase and neosubstrates. Proc. Natl. Acad. Sci. U.S.A. Mar 20 2017 [PMID: 28320958] (WB, Human) WB Human
Gao S, Geng C, Song T et al. Activation of c-Abl kinase potentiates the anti-myeloma drug lenalidomide by promoting DDA1 recruitment to the CRL4 ubiquitin ligase J. Biol. Chem Jan 13 2017 [PMID: 28087699]
Zhu YX, Braggio E, Shi CX et al. Identification of cereblon binding proteins and relationship with response and survival following pomalidomide and dexamethasone in multiple myeloma. Blood. 2014 Jun 09 [PMID: 24914135] (WB, Human)

Details:
Figs 3 & 5: IKZF1, IKZF3 or cerebron (CRBN), lentiviral infected human myeloma cell lines (HMCLs) and infected HMCLs treated with IKZF1, IKZF3 or CRBN ShRNAs, respecitvely. The specificity of the IKZF3 pAb was validated in IKZF3-infected HMCLs and in IKZF3-shRNA infected cells by WB (Figs 3 & 5). The IKZF3 pAb recognized IKZF3 in WB that had been co-IP'd using a CRBN antibody, thereby helping to demonstrate that IKZF3 is a CRNB binding protein (Fig 3D). 		WB Human

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Bioinformatics

Gene Symbol IKZF3
Uniprot