Galectin-7 Antibody (212923) [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: WB, IHC, ELISA(Cap), ELISA(Det)
• Clone: 212923
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 594

Galectin-7 Antibody (212923) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant mouse Galectin‑7
  Ser2-Phe136
  Accession # AAK29385
• Specificity: Detects mouse Galectin-7 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Galectin‑1, ‑3, ‑4, recombinant human (rh) Galectin-2, -7, or -8 is observed. In Western blots, approximately 20% cross-reactivi
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-7 Antibody (212923) [Alexa Fluor® 594]

• GAL7
• Gal-7
• galectin 7
• Galectin7
• Galectin-7
• HKL-14
• lectin, galactoside-binding, soluble, 7
• LGALS7
• LGALS7A
• p53-induced gene 1 protein
• PI7
• PIG1
• TP53I1

Background

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1‑4).

Mouse Galectin-7 is a prototype monomeric galectin. It is expressed in stratified epithelia and is significantly down-regulated in squamous cell carcinomas. Galectin-7 is a pro-apoptotic protein that is highly induced by the tumor suppressor protein p53. It functions intracellularly upstream of JNK activation to enhance cytochrome c release during apoptosis (5). Galectin-7 may also be involved in cell-cell and cell-matrix interactions and exogenous galectin has been found to accelerate the re‑epithelialization of wounds (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.