GALT Antibody (4C11)

Images

 
Western Blot: GALT Antibody (4C11) [H00002592-M01] - GALT monoclonal antibody (M01), clone 4C11 Analysis of GALT expression in K-562.
Immunohistochemistry-Paraffin: GALT Antibody (4C11) [H00002592-M01] - Analysis of monoclonal antibody to GALT on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 1 ug/ml.
Western Blot: GALT Antibody (4C11) [H00002592-M01] - Analysis of GALT expression in transfected 293T cell line by GALT monoclonal antibody (M01), clone 4C11.Lane 1: GALT transfected lysate(43 KDa).Lane 2: ...read more
Sandwich ELISA: GALT Antibody (4C11) [H00002592-M01] - Detection limit for recombinant GST tagged GALT is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, IHC-P
Clone
4C11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

GALT Antibody (4C11) Summary

Immunogen
GALT (AAH15045, 1 a.a. - 379 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSRSGTDPQQRQQASEADAAAATFRANDHQHIRYNPLQDEWVLVSAHRMKRPWQGQVEPQLLKTVPRHDPLNPLCPGAIRANGEVNPQYDSTFLFDNDFPALQPDAPSPGPSDHPLFQAKSARGVCKVMCFHPWSDVTLPLMSVPEIRAVVDAWASVTEELGAQYPWVQIFENKGAMMGCSNPHPHCQVWASSFLPDIAQREERSQQAYKSQHGEPLLMEYSRQELLRKERLVLTSEHWLVLVPFWATWPYQTLLLPRRHVRRLPELTPAERDDLASIMKKLLTKYDNLFETSFPYSMGWHGAPTGSEAGANWDHWQLHAHYYPPLLRSATVRKFMVGYEMLAQAQRDLTPEQAAERLRALPEVHYHLGQKDRETATIA
Specificity
GALT - galactose-1-phosphate uridylyltransferase
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GALT
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry-Paraffin
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IHC-P and ELISA.
Reviewed Applications
Read 1 Review rated 5
using
H00002592-M01 in the following applications:

Reactivity Notes

Rat reactivity reported from customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GALT Antibody (4C11)

  • EC 2.7.7.12
  • Gal-1-P uridylyltransferase
  • galactose-1-phosphate uridyl transferase
  • galactose-1-phosphate uridylyltransferase
  • UDP-glucose--hexose-1-phosphate uridylyltransferase

Background

Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GALT Antibody (H00002592-M01) (0)

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Review for GALT Antibody (H00002592-M01) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: WB.

Reviews using H00002592-M01:
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Other 12/21/2009
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Summary

ApplicationWestern Blot
Sample TestedColumn fractions, Sample Amount: 100 ng
SpeciesOther

Blocking

Blocking DetailsBlocking Buffer: 5% blotto, Blocking Time: 45 minutes, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1/5000, Incubation Dilution Buffer: 2.5% blotto, Incubation Time: 45 minutes, Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti mouse HRP pAb, Secondary Ab Dilution Ratio: 1/10000
Secondary Manufacturer Cat#not used

Details

Detection NotesDetection Method: ECL Hyperfilm, Exposure Time: 1 minute, Positive Control: Load, Specific Bands: 55 kDa

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for GALT Antibody (H00002592-M01) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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Anonymous
12/21/2009
Application: WB
Species: Other

Bioinformatics

Gene Symbol GALT
Entrez
OMIM
Uniprot