Rat Galectin-4 ELISA Kit (Colorimetric)

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ELISA: Rat Galectin-4 ELISA Kit (Colorimetric) [NBP2-76726] - Samples were spiked with high concentrations of Rat Galectin-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Rat Galectin-4 ELISA Kit (Colorimetric) [NBP2-76726] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.156 - 10ng/mL
Sensitivity
0.094ng/mL

Order Details

Rat Galectin-4 ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.156 - 10ng/mL
Sensitivity
0.094ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.7
Intra-Assay
CV% < 5.44
Spike Recovery
92-108%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
LGALS4

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Galectin-4 ELISA Kit (Colorimetric)

  • Antigen NY-CO-27
  • GAL4
  • gal-4
  • galectin 4
  • Galectin4
  • Galectin-4
  • L-36 lactose-binding protein
  • L36LBP
  • Lactose-binding lectin 4
  • lectin, galactoside-binding, soluble, 4
  • LGALS4

Background

Galectins are a family of soluble beta-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. One member of this family, Galectin-4, also known as Gal-4, L36 or LGALS4 maps to human chromosome 19q13.13 and encodes a 36-37 kDa protein. The Galectin-4 protein is composed of 323 amino acids and contains two homologous carbohydrate recognition domains (CRD) and all amino acids typically conserved in the galectin family. Expression of Galectin-4 correlates with the malignant potential of human hepatocellular carcinoma (HCC) and is differentially regulated depending on cell-cell contact, serum growth factors, cell growth and cell differentiation status. Galectin-4 expression is detected in epithelial cells of the colon, rectum, intestine, and in HT29 and LS174T cell lines. Galectin-4 is underexpressed in colorectal cancer and is preferentially upregulated in cells prone to peritoneal dissemination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol LGALS4