Mouse Galectin-4 ELISA Kit (Colorimetric)

ELISA: Mouse Galectin-4 ELISA Kit (Colorimetric) [NBP2-76725] - Samples were spiked with high concentrations of Mouse Galectin-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Galectin-4 ELISA Kit (Colorimetric) [NBP2-76725] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.156 - 10ng/mL
• Sensitivity: 0.094ng/mL

Mouse Galectin-4 ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 0.156 - 10ng/mL
• Sensitivity: 0.094ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.72
• Intra-Assay: CV% < 5.2
• Spike Recovery: 86-108%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: LGALS4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Galectin-4 ELISA Kit (Colorimetric)

• Antigen NY-CO-27
• GAL4
• gal-4
• galectin 4
• Galectin4
• Galectin-4
• L-36 lactose-binding protein
• L36LBP
• Lactose-binding lectin 4
• lectin, galactoside-binding, soluble, 4
• LGALS4

Background

Galectins are a family of soluble beta-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. One member of this family, Galectin-4, also known as Gal-4, L36 or LGALS4 maps to human chromosome 19q13.13 and encodes a 36-37 kDa protein. The Galectin-4 protein is composed of 323 amino acids and contains two homologous carbohydrate recognition domains (CRD) and all amino acids typically conserved in the galectin family. Expression of Galectin-4 correlates with the malignant potential of human hepatocellular carcinoma (HCC) and is differentially regulated depending on cell-cell contact, serum growth factors, cell growth and cell differentiation status. Galectin-4 expression is detected in epithelial cells of the colon, rectum, intestine, and in HT29 and LS174T cell lines. Galectin-4 is underexpressed in colorectal cancer and is preferentially upregulated in cells prone to peritoneal dissemination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Galectin-4 ELISA Kit (NBP2-76725)(1)

Publication using NBP2-76725

• Jujic A, Vieira JPP, Matuskova H et al. Plasma Galectin-4 Levels Are Increased after Stroke in Mice and Humans International journal of molecular sciences 2023-06-13 [PMID: 37373212] (ELISA)

FAQs for Galectin-4 ELISA Kit (NBP2-76725). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: LGALS4