GABA Transporter 2 Antibody - Azide Free

Western Blot: GABA Transporter 2 Antibody [NB100-61098] - Analysis of mouse whole brain lysate showing specific immunolabeling of the ~67 kDa GAT-2 protein.

• Reactivity: Mu, Rt, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

GABA Transporter 2 Antibody - Azide Free Summary

• Immunogen: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Accession # P31646
• Specificity: Specific for endogenous levels of the ~82 kDa GABA Transporter 2 protein.
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC6A13
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-200
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Affinity purified

Alternate Names for GABA Transporter 2 Antibody - Azide Free

• GABA transport protein
• GAT-2
• GAT2Solute carrier family 6 member 13
• sodium- and chloride-dependent GABA transporter 2
• solute carrier family 6 (neurotransmitter transporter, GABA), member 13

Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl negative channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high-affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-2 is found in a wide range of neuronal and non-neuronal cells including dendrites and axon terminals as well as epithelial cells and cells forming the pia and arachnoid complex (Conti et al., 1999).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC6A13
• Entrez:
  • 171163(Rat)
• Uniprot:
  • P31646(Rat)