GABA-A R delta Antibody (N151/3) - BSA Free

Western Blot: GABA-A R delta Antibody (N151/3) [NBP1-47616] - Western Blot analysis of Rat Cell line lysates showing detection of GABA-A R delta protein using Mouse Anti-GABA-A R delta Monoclonal Antibody, Clone N151/3 (NBP1-47616). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA-A R delta Monoclonal Antibody (NBP1-47616) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: GABA-A R delta Antibody (N151/3) [NBP1-47616] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Delta Monoclonal Antibody, Clone N151/3 (NBP1-47616). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Delta Monoclonal Antibody (NBP1-47616) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Delta Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: GABA-A R delta Antibody (N151/3) [NBP1-47616] - analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S151-3 . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Diffuse cytoplasm and dull nuclei.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, MA
• Clone: N151/3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GABA-A R delta Antibody (N151/3) - BSA Free Summary

• Description: Novus Biologicals Mouse GABA-A R delta Antibody (N151/3) - BSA Free (NBP1-47616) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide amino acids 15-34 (N-terminus) of rat GABA-A-R-Delta
• Localization: Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane
• Specificity: Detects approx 55kDa.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GABRD
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:1000
  • Microarray
  • Western Blot 1:1000
• Application Notes: 2 ug/ml of GABA-A Receptor Delta Antibody was sufficient for detection of Delta1 GABA-A receptor in 10 ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for GABA-A R delta Antibody (N151/3) - BSA Free

• EIG10
• EJM7
• GABA A R delta
• GABA(A) receptor subunit delta
• GABA-A receptor, delta polypeptide
• GABAAR delta
• GABAARd
• GABRD
• gamma-aminobutyric acid (GABA) A receptor, delta
• gamma-aminobutyric acid receptor subunit delta
• GEFSP5
• MGC45284

Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six as, four bs and four gs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for a- and b-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a g-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different a-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Poltl et al., 2003). More recently there have been a number of studies demonstrating that the alpha-subunit of the receptor may affect subunit assembly (Korpi et al., 2002) and may also confer differential sensitivity to neurosteroids and to ethanol (Wallner et al., 2003; Wohlfarth et al., 2002).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GABRD
• Entrez:
  • 29689(Rat)
• Uniprot:
  • P18506(Rat)