GABA-A R delta Antibody (N151/3) [DyLight 650]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: N151/3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 650

GABA-A R delta Antibody (N151/3) [DyLight 650] Summary

• Immunogen: Synthetic peptide amino acids 15-34 (N-terminus) of rat GABA-A-R-Delta
• Localization: Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane
• Specificity: Detects approx 55kDa.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GABRD
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for GABA-A R delta Antibody (N151/3) [DyLight 650]

• EIG10
• EJM7
• GABA A R delta
• GABA(A) receptor subunit delta
• GABA-A receptor, delta polypeptide
• GABAAR delta
• GABAARd
• GABRD
• gamma-aminobutyric acid (GABA) A receptor, delta
• gamma-aminobutyric acid receptor subunit delta
• GEFSP5
• MGC45284

Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six as, four bs and four gs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for a- and b-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a g-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different a-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; P ltl et al., 2003). More recently there have been a number of studies demonstrating that the -subunit of the receptor may affect subunit assembly (Korpi et al., 2002) and may also confer differential sensitivity to neurosteroids and to ethanol (Wallner et al., 2003; Wohlfarth et al., 2002).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GABRD
• Entrez:
  • 29689(Rat)
• Uniprot:
  • P18506(Rat)