G3BP2 Antibody

Images

 
Western Blot: G3BP2 Antibody [NBP1-82977] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: G3BP2 Antibody [NBP1-82977] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: G3BP2 Antibody [NBP1-82977] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: G3BP2 Antibody [NBP1-82977] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: G3BP2 Antibody [NBP1-82977] - Staining of human skin shows low expression as expected.
Immunohistochemistry-Paraffin: G3BP2 Antibody [NBP1-82977] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemistry-Paraffin: G3BP2 Antibody [NBP1-82977] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells and cells in molecular layer.
Immunohistochemistry-Paraffin: G3BP2 Antibody [NBP1-82977] - Staining in human cerebral cortex and skin tissues using anti-G3BP2 antibody. Corresponding G3BP2 RNA-seq data are presented for the same tissues.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

G3BP2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: HNDMFRYEDEVFGDSEPELDEESEDEVEEEQEERQPSPEPVQENANSGYYEAHPVTNGIEEPLEESSHEPEPEPESETKTEELKPQVE
Specificity
Specificity of human, mouse, rat G3BP2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
G3BP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
G3BP2 Protein (NBP1-82977PEP)
Publications
Read Publications using
NBP1-82977 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 22689141).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for G3BP2 Antibody

  • G3BP-2
  • GAP SH3 domain-binding protein 2
  • GTPase activating protein (SH3 domain) binding protein 2
  • KIAA0660
  • ras GTPase-activating protein-binding protein 2
  • Ras-GTPase activating protein SH3 domain-binding protein 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for G3BP2 Antibody (NBP1-82977)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
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Filter By Species
Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol G3BP2