ABCA2 Antibody

Immunohistochemistry-Paraffin: ABCA2 Antibody [NBP1-20863] - (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ABCA2 Antibody Summary

• Description: Novus Biologicals Goat ABCA2 Antibody (NBP1-20863) is a polyclonal antibody validated for use in IHC, WB, ELISA and IP. Anti-ABCA2 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KKQSDNLEQQETEP corresponding to internal region according to NP_997698.1.
• Epitope: C terminus
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001597.2; NP_997698.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ABCA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 0.3 ug/ml
• Application Notes: Western blot: Preliminary experiments gave an approx 35kDa band in Human Brain (Amygdala, Frontal Cortex, Hippocampus and Substantia Nigra) lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 270kDa according to NP_001597.2 and of 273kDa according to .NP_997698.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide.
  IHC: Paraffin embedded Human Brain (Cortex). Use in immunoprecipitation reported in scientific literature (PMID:31334335).

Reactivity Notes

Expected from sequence similarity: Mouse and Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ABCA2 Antibody

• ABC2ATP-binding cassette, sub-family A, member 2
• ATP-binding cassette 2
• ATP-binding cassette sub-family A member 2
• ATP-binding cassette transporter 2
• ATP-binding cassette, sub-family A (ABC1), member 2
• EC 3.6.3
• EC 3.6.3.41
• KIAA1062
• MGC129761

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein is highly expressed in brain tissue and may play a role in macrophage lipid metabolism and neural development. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCA2 Antibody (NBP1-20863)(3)

Publications using NBP1-20863

• A Po, A Citarella, G Catanzaro, ZM Besharat, S Trocchiane, F Gianno, C Sabato, M Moretti, E De Smaele, A Vacca, ME Fiori, E Ferretti Hedgehog-GLI signalling promotes chemoresistance through the regulation of ABC transporters in colorectal cancer cells Sci Rep, 2020-08-19;10(1):13988. 2020-08-19 [PMID: 32814794] (WB, Human)
• Huang C, Zhang X, Jiang L et al. FoxM1 Induced Paclitaxel Resistance via Activation of the FoxM1/PHB1/RAF-MEK-ERK Pathway and Enhancement of the ABCA2 Transporter Mol Ther Oncolytics 2019-09-27 [PMID: 31334335] (WB, IP, Human)
• Mace S, Cousin E, Ricard S et al. ABCA2 is a strong genetic risk factor for early-onset Alzheimer's disease Neurobiol Dis 2005-02-01 [PMID: 15649702]

Bioinformatics

• Gene Symbol: ABCA2
• Entrez:
  • 20(Human)
  • 79248(Rat)
• Uniprot:
  • NP_001597.2(Human)