ABCA2 Antibody

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Immunohistochemistry-Paraffin: ABCA2 Antibody [NBP1-20863] - (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ABCA2 Antibody Summary

Immunogen
Peptide with sequence C-KKQSDNLEQQETEP corresponding to internal region according to NP_997698.1.
Epitope
C terminus
Specificity
This antibody is expected to recognize both reported isoforms (NP_001597.2; NP_997698.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ABCA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:2000
Application Notes
Western blot: Preliminary experiments gave an approx 35kDa band in Human Brain (Amygdala, Frontal Cortex, Hippocampus and Substantia Nigra) lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 270kDa according to NP_001597.2 and of 273kDa according to.NP_997698.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Brain (Cortex). Use in immunoprecipitation reported in scientific literature (PMID:31334335).
Publications
Read Publications using
NBP1-20863 in the following applications:

  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

Expected from sequence similarity: Mouse and Rat

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for ABCA2 Antibody

  • ABC2ATP-binding cassette, sub-family A, member 2
  • ATP-binding cassette 2
  • ATP-binding cassette sub-family A member 2
  • ATP-binding cassette transporter 2
  • ATP-binding cassette, sub-family A (ABC1), member 2
  • EC 3.6.3
  • EC 3.6.3.41
  • KIAA1062
  • MGC129761

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein is highly expressed in brain tissue and may play a role in macrophage lipid metabolism and neural development. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ABCA2 Antibody (NBP1-20863)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


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IP
(1)
WB
(2)
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Human
(2)
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Showing Publications 1 - 3 of 3.
Publications using NBP1-20863 Applications Species
Morwitzer MJ, Tritsch SR, Cazares LH et al. FoxM1 Induced Paclitaxel Resistance via Activation of FoxM1/PHB1/RAF-MEK-ERK Pathway and Enhancement of ABCA2 Transporter Molecular Therapy - Oncolytics May 1 2019 (WB, Human) WB Human
Huang C, Zhang X, Jiang L et al. FoxM1 Induced Paclitaxel Resistance via Activation of the FoxM1/PHB1/RAF-MEK-ERK Pathway and Enhancement of the ABCA2 Transporter Mol Ther Oncolytics Sep 27 2019 [PMID: 31334335] (IP, WB, Human) IP, WB Human
Mace S, Cousin E, Ricard S et al. ABCA2 is a strong genetic risk factor for early-onset Alzheimer's disease Neurobiol Dis 2005 Feb [PMID: 15649702]

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Product General Protocols

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Bioinformatics

Gene Symbol ABCA2
Entrez
Uniprot