FA2H Antibody (OTI2E3)

Western Blot: FA2H Antibody (OTI2E3) [NBP2-45564] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FA2H.

Immunofluorescence: FA2H Antibody (OTI2E3) [NBP2-45564] - Analysis of COS7 cells transiently transfected with pCMV6-ENTRY FA2H.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC-P, ICC/IF
• Clone: OTI2E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

FA2H Antibody (OTI2E3) Summary

• Immunogen: Full length human recombinant protein of human FA2H(NP_077282) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FA2H
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunofluorescence 1:50-1:100
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Theoretical MW: 42.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FA2H Antibody (OTI2E3)

• FAAHFAXDC1
• FAH1
• fatty acid 2-hydroxylase
• Fatty acid alpha-hydroxylase
• fatty acid hydroxylase domain containing 1
• FLJ25287
• SCS7
• spastic paraplegia 35 (autosomal recessive)
• SPG35

Background

Sphingolipids are a large class of lipids found in all eukaryotic cells and are involved in numerous cellular processes. The structural diversity of sphingolipids stems from more than 300 distinct head groups, as well as from modifications of the hydrophobic ceramide moiety. FA2H catalyzes a common modification of the ceramide moiety: hydroxylation at the 2 position of the N-acyl chain. Sphingolipids containing 2-hydroxy fatty acid are common in nervous and epidermal tissue. Glycosphingolipids containing a high proportion of 2-hydroxy fatty acid are critical components of myelin, and several very long chain ceramides with 2-hydroxy fatty acids are important for the permeability barrier function of epidermis (Alderson et al., 2004 [PubMed 15337768]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FA2H