Western Blot: GPIP137 Antibody [NBP2-22238] - analysis of GPIP137 in human B-cell whole cell lysate using GPIP137 antibody. Image from verified customer review.
Biological Strategies: Western Blot: GPIP137 Antibody [NBP2-22238] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22238 used for WB at 0.1 ...read more
Independent Antibodies: Immunoprecipitation: GPIP137 Antibody [NBP2-22238] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: NBP2-22238 used for IP at 6 ug/mg lysate. GPIP137 ...read more
The signal was strong, however; it showed up in the entire cell. Other CAPRIN1 IF images had no signal in the nucleus. So, it worked, but there are probably are antibodies validated for IF that should be compared side by side with this one to confirm. Diluted 1:250 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation.
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