ROR1 Antibody [Unconjugated] (AF2000)

Images

 
Western blot shows lysate of NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000) ...read more
Simple Western lane view shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ROR1 at approximately 143 kDa (as indicated) using 25 ...read more
MDA‑MB‑231 human breast cancer cell line was stained with Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

ROR1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROR1
Ala24-Glu403
Accession # Q01973
Specificity
Detects human ROR1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human ROR2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ROR1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 25 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF2000 in the following applications:

Publications
Read Publications using
AF2000 in the following applications:

  • 2 publications
  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROR1 Antibody [Unconjugated]

  • EC 2.7.10.1
  • MGC99659
  • neurotrophic tyrosine kinase receptor-related 1
  • Neurotrophic tyrosine kinase, receptor-related 1
  • NTRKR1
  • NTRKR1dJ537F10.1
  • receptor tyrosine kinase-like orphan receptor 1
  • ROR1
  • tyrosine-protein kinase transmembrane receptor ROR1

Background

ROR1 belongs to the ROR family of receptor tyrosine kinases, which are characterized by: the extracellular immunoglobulin domain, cysteine-rich domain that resembles the Wnt-binding sites of Frizzled proteins, kringle domain and intracellular kinase domain. Human ROR1 shares 97% and 58% amino acid sequence identity with mouse ROR1 and human ROR2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ROR1 Antibody (AF2000)(4)

Reviews for ROR1 Antibody (AF2000) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 application: WB, Flow.

Reviews using AF2000:
Filter by Applications
WB
(1)
Flow
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23593420
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23593420

Details

Detection NotesSee PMID 23593420

Comments

CommentsExcellent ABSee PMID 23593420
 
reviewed by:
Anonymous
Flow Human 01/06/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 20597086
SpeciesHuman

Blocking

Blocking DetailsSee PMID 20597086

Details

Detection NotesSee PMID 20597086

Comments

CommentsPublished: See PMID 20597086

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ROR1 Antibody (AF2000) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Blogs on ROR1

There are no specific blogs for ROR1, but you can read our latest blog posts.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Anonymous
01/06/2015
Application: WB
Species: Human

 
Anonymous
01/06/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol ROR1
Entrez
Uniprot