ROR1 Antibody [Unconjugated]

Western blot shows lysate of NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ROR1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MDA‑MB‑231 human breast cancer cell line was stained with Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Simple Western lane view shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ROR1 at approximately 143 kDa (as indicated) using 25 µg/mL of Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ROR1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ROR1
  Ala24-Glu403
  Accession # Q01973
• Specificity: Detects human ROR1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ROR1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROR1 Antibody [Unconjugated]

• EC 2.7.10.1
• MGC99659
• neurotrophic tyrosine kinase receptor-related 1
• Neurotrophic tyrosine kinase, receptor-related 1
• NTRKR1
• NTRKR1dJ537F10.1
• receptor tyrosine kinase-like orphan receptor 1
• ROR1
• tyrosine-protein kinase transmembrane receptor ROR1

Background

ROR1 belongs to the ROR family of receptor tyrosine kinases, which are characterized by: the extracellular immunoglobulin domain, cysteine-rich domain that resembles the Wnt-binding sites of Frizzled proteins, kringle domain and intracellular kinase domain. Human ROR1 shares 97% and 58% amino acid sequence identity with mouse ROR1 and human ROR2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ROR1 Antibody (AF2000)(15)

Publications using AF2000

• D Liu, GF Kaufmann, JB Breitmeyer, KA Dickson, DJ Marsh, CE Ford The Anti-ROR1 Monoclonal Antibody Zilovertamab Inhibits the Proliferation of Ovarian and Endometrial Cancer Cells Pharmaceutics, 2022;14(4):. 2022-01-01 [PMID: 35456672] (Western Blot, Human)
• Y Liu, H Deng, L Liang, G Zhang, J Xia, K Ding, N Tang, K Wang Depletion of VPS35 attenuates metastasis of hepatocellular carcinoma by restraining the Wnt/PCP signaling pathway Genes & diseases, 2021;8(2):232-240. 2021-01-01 [PMID: 33997170] (Western Blot, Human)
• D Liu, K Gunther, LA Enriquez, B Daniels, TA O'Mara, K Tang, AB Spurdle, CE Ford ROR1 is upregulated in endometrial cancer and represents a novel therapeutic target Sci Rep, 2020;10(1):13906. 2020-01-01 [PMID: 32807831] (Western Blot, Human)
• SS Islam, M Uddin, ASM Noman, H Akter, NJ Dity, M Basiruzzma, F Uddin, J Ahsan, S Annoor, AA Alaiya, M Al-Alwan, H Yeger, WA Farhat Antibody-drug conjugate T-DM1 treatment for HER2+ breast cancer induces ROR1 and confers resistance through activation of Hippo transcriptional coactivator YAP1 EBioMedicine, 2019;0(0):. 2019-01-01 [PMID: 31085100] (Western Blot, Human)
• T Yamaguchi, M Hayashi, L Ida, M Yamamoto, C Lu, T Kajino, J Cheng, M Nakatochi, H Isomura, M Yamazaki, M Suzuki, T Fujimoto, T Takahashi ROR1-CAVIN3 interaction required for caveolae-dependent endocytosis and pro-survival signaling in lung adenocarcinoma Oncogene, 2019;0(0):. 2019-01-01 [PMID: 30894682] (Immunoprecipitation, Human)
• AH Daneshmane, M Hojjat-Far, A Ghaderi, A Moshfegh, L Hansson, J Schultz, J Vågberg, S Byström, E Olsson, T Olin, A Österborg, H Mellstedt A receptor tyrosine kinase ROR1 inhibitor (KAN0439834) induced significant apoptosis of pancreatic cells which was enhanced by erlotinib and ibrutinib PLoS ONE, 2018;13(6):e0198038. 2018-01-01 [PMID: 29856777] (Western Blot, Human)
• M Chow, L Gao, JD MacManiman, VT Bicocca, BH Chang, JJ Alumkal, JW Tyner Maintenance and pharmacologic targeting of ROR1 protein levels via UHRF1 in t(1;19) pre-B-ALL Oncogene, 2018;0(0):. 2018-01-01 [PMID: 29849118] (Immunoprecipitation, Human)
• CE Henry, E Llamosas, B Daniels, A Coopes, K Tang, CE Ford ROR1 and ROR2 play distinct and opposing roles in endometrial cancer Gynecol. Oncol., 2018;0(0):. 2018-01-01 [PMID: 29395309] (Western Blot, Human)
• C Henry, N Hacker, C Ford Silencing ROR1 and ROR2 inhibits invasion and adhesion in an organotypic model of ovarian cancer metastasis Oncotarget, 2017;8(68):112727-112738. 2017-01-01 [PMID: 29348860] (Western Blot, Human)
• H Karvonen, K Summala, W Niininen, HR Barker, D Ungureanu Interaction between ROR1 and MuSK activation complex in myogenic cells FEBS Lett., 2018;0(0):. 2018-01-01 [PMID: 29292499] (Western Blot, Human)
• T Yamaguchi, C Lu, L Ida, K Yanagisawa, J Usukura, J Cheng, N Hotta, Y Shimada, H Isomura, M Suzuki, T Fujimoto, T Takahashi ROR1 sustains caveolae and survival signalling as a scaffold of cavin-1 and caveolin-1 Nat Commun, 2016;7(0):10060. 2016-01-01 [PMID: 26725982] (Immunoprecipitation, Human)
• Henry C, Llamosas E, Knipprath-Meszaros A, Schoetzau A, Obermann E, Fuenfschilling M, Caduff R, Fink D, Hacker N, Ward R, Heinzelmann-Schwarz V, Ford C Targeting the ROR1 and ROR2 receptors in epithelial ovarian cancer inhibits cell migration and invasion. Oncotarget, 0;6(37):40310-26. [PMID: 26515598] (Western Blot, Human)
• Gentile A, Lazzari L, Benvenuti S, Trusolino L, Comoglio PM Ror1 is a pseudokinase that is crucial for met-driven tumorigenesis. Cancer Res., 2011;71(8):3132-41. 2011-01-01 [PMID: 21487037] (Immunoprecipitation, Human)
• Hudecek M, Schmitt TM, Baskar S The B-cell tumor-associated antigen ROR1 can be targeted with T cells modified to express a ROR1-specific chimeric antigen receptor. Blood, 2010;116(22):4532-41. 2010-01-01 [PMID: 20702778] (Flow Cytometry, Human)
• Baskar S, Kwong KY, Hofer T, Levy JM, Kennedy MG, Lee E, Staudt LM, Wilson WH, Wiestner A, Rader C Unique cell surface expression of receptor tyrosine kinase ROR1 in human B-cell chronic lymphocytic leukemia. Clin. Cancer Res., 2008;14(2):396-404. 2008-01-01 [PMID: 18223214] (Flow Cytometry, Human)

Reviews for ROR1 Antibody (AF2000) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Anonymous - WB Human 01/06/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23593420
• Species: Human

reviewed by: Anonymous - Flow Human 01/06/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 20597086
• Species: Human

Bioinformatics

• Gene Symbol: ROR1
• Uniprot:
  • Q01973()