ROR1 Antibody [Unconjugated]

Images

 
Western blot shows lysate of NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000) ...read more
MDA‑MB‑231 human breast cancer cell line was stained with Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000, filled histogram) or isotype control antibody (Catalog # ...read more
Simple Western lane view shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ROR1 at approximately 143 kDa (as indicated) using 25 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

ROR1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROR1
Ala24-Glu403
Accession # Q01973
Specificity
Detects human ROR1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human ROR2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ROR1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 25 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF2000 in the following applications:

Publications
Read Publications using
AF2000 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROR1 Antibody [Unconjugated]

  • EC 2.7.10.1
  • MGC99659
  • neurotrophic tyrosine kinase receptor-related 1
  • Neurotrophic tyrosine kinase, receptor-related 1
  • NTRKR1
  • NTRKR1dJ537F10.1
  • receptor tyrosine kinase-like orphan receptor 1
  • ROR1
  • tyrosine-protein kinase transmembrane receptor ROR1

Background

ROR1 belongs to the ROR family of receptor tyrosine kinases, which are characterized by: the extracellular immunoglobulin domain, cysteine-rich domain that resembles the Wnt-binding sites of Frizzled proteins, kringle domain and intracellular kinase domain. Human ROR1 shares 97% and 58% amino acid sequence identity with mouse ROR1 and human ROR2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ROR1 Antibody (AF2000)(12)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow Cytometry, Immunoprecipitation, Western Blot.


Filter By Application
Flow Cytometry
(2)
Immunoprecipitation
(4)
Western Blot
(6)
All Applications
Filter By Species
Human
(12)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF2000 Applications Species
SS Islam, M Uddin, ASM Noman, H Akter, NJ Dity, M Basiruzzma, F Uddin, J Ahsan, S Annoor, AA Alaiya, M Al-Alwan, H Yeger, WA Farhat Antibody-drug conjugate T-DM1 treatment for HER2+ breast cancer induces ROR1 and confers resistance through activation of Hippo transcriptional coactivator YAP1 EBioMedicine, 2019;0(0):. 2019 [PMID: 31085100] (Western Blot, Human) Western Blot Human
T Yamaguchi, M Hayashi, L Ida, M Yamamoto, C Lu, T Kajino, J Cheng, M Nakatochi, H Isomura, M Yamazaki, M Suzuki, T Fujimoto, T Takahashi ROR1-CAVIN3 interaction required for caveolae-dependent endocytosis and pro-survival signaling in lung adenocarcinoma Oncogene, 2019;0(0):. 2019 [PMID: 30894682] (Immunoprecipitation, Human) Immunoprecipitation Human
AH Daneshmane, M Hojjat-Far, A Ghaderi, A Moshfegh, L Hansson, J Schultz, J Vågberg, S Byström, E Olsson, T Olin, A Österborg, H Mellstedt A receptor tyrosine kinase ROR1 inhibitor (KAN0439834) induced significant apoptosis of pancreatic cells which was enhanced by erlotinib and ibrutinib PLoS ONE, 2018;13(6):e0198038. 2018 [PMID: 29856777] (Western Blot, Human) Western Blot Human
M Chow, L Gao, JD MacManiman, VT Bicocca, BH Chang, JJ Alumkal, JW Tyner Maintenance and pharmacologic targeting of ROR1 protein levels via UHRF1 in t(1;19) pre-B-ALL Oncogene, 2018;0(0):. 2018 [PMID: 29849118] (Immunoprecipitation, Human) Immunoprecipitation Human
CE Henry, E Llamosas, B Daniels, A Coopes, K Tang, CE Ford ROR1 and ROR2 play distinct and opposing roles in endometrial cancer Gynecol. Oncol., 2018;0(0):. 2018 [PMID: 29395309] (Western Blot, Human) Western Blot Human
C Henry, N Hacker, C Ford Silencing ROR1 and ROR2 inhibits invasion and adhesion in an organotypic model of ovarian cancer metastasis Oncotarget, 2017;8(68):112727-112738. 2017 [PMID: 29348860] (Western Blot, Human) Western Blot Human
H Karvonen, K Summala, W Niininen, HR Barker, D Ungureanu Interaction between ROR1 and MuSK activation complex in myogenic cells FEBS Lett., 2018;0(0):. 2018 [PMID: 29292499] (Western Blot, Human) Western Blot Human
T Yamaguchi, C Lu, L Ida, K Yanagisawa, J Usukura, J Cheng, N Hotta, Y Shimada, H Isomura, M Suzuki, T Fujimoto, T Takahashi ROR1 sustains caveolae and survival signalling as a scaffold of cavin-1 and caveolin-1 Nat Commun, 2016;7(0):10060. 2016 [PMID: 26725982] (Immunoprecipitation, Human) Immunoprecipitation Human
Henry C, Llamosas E, Knipprath-Meszaros A, Schoetzau A, Obermann E, Fuenfschilling M, Caduff R, Fink D, Hacker N, Ward R, Heinzelmann-Schwarz V, Ford C Targeting the ROR1 and ROR2 receptors in epithelial ovarian cancer inhibits cell migration and invasion. Oncotarget, 0;6(37):40310-26. 0 [PMID: 26515598] (Western Blot, Human) Western Blot Human
Gentile A, Lazzari L, Benvenuti S, Trusolino L, Comoglio PM Ror1 is a pseudokinase that is crucial for met-driven tumorigenesis. Cancer Res., 2011;71(8):3132-41. 2011 [PMID: 21487037] (Immunoprecipitation, Human) Immunoprecipitation Human
Show All 12 Publications.

Reviews for ROR1 Antibody (AF2000) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF2000:
Filter by Applications
WB
(1)
Flow
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23593420
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23593420

Details

Detection NotesSee PMID 23593420

Comments

CommentsExcellent ABSee PMID 23593420
 
reviewed by:
Anonymous
Flow Human 01/06/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 20597086
SpeciesHuman

Blocking

Blocking DetailsSee PMID 20597086

Details

Detection NotesSee PMID 20597086

Comments

CommentsPublished: See PMID 20597086

Product General Protocols

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Video Protocols

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FAQs for ROR1 Antibody (AF2000) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 FAB2000G
Biotin BAF2000
Phycoerythrin FAB2000P

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Bioinformatics Tool for ROR1 Antibody (AF2000)

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Blogs on ROR1

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
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Anonymous
01/06/2015
Application: WB
Species: Human

 
Anonymous
01/06/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol ROR1
Uniprot