FUS Antibody

Images

 
Genetic Strategies: Knockout Validated: FUS Antibody [NB100-2599] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. ...read more
Immunohistochemistry-Paraffin: FUS Antibody [NB100-2599] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-FUS used at a dilution of 1:100. Detection: DAB
Immunohistochemistry-Paraffin: FUS Antibody [NB100-2599] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FUS used at a dilution of 1:500. Detection: DAB staining using anti-rabbit IHC ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Mk, Pm, RbSpecies Glossary
Applications WB, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

FUS Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human fusion (involved in t(12;16) in malignant liposarcoma) using the numbering given in SwissProt entry P35637 (GeneID 2521).
Predicted Species
Rat (100%), Porcine (100%), Rabbit (100%), Bovine (100%), Canine (100%), Monkey (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FUS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Knockout Validated
Application Notes
Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections.
Control
FUS Overexpression Lysate
FUS Knockout 293T Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-2599 in the following applications:

Publications
Read Publications using
NB100-2599 in the following applications:

  • IHC
    1 publication
  • 2 publications
  • WB
    1 publication

Reactivity Notes

Panda, Orangutan, Monkey, Gorilla, Chimpanzee and hirteen-lined ground squirrel (100%).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FUS Antibody

  • 75 kDa DNA-pairing protein
  • ALS6
  • amyotrophic lateral sclerosis 6
  • FUS1
  • fused in sarcoma
  • fusion (involved in t(12;16) in malignant liposarcoma)
  • fusion gene in myxoid liposarcoma
  • fusion, derived from t(12;16) malignant liposarcoma
  • fus-like protein
  • heterogeneous nuclear ribonucleoprotein P2
  • HNRNPP2
  • Oncogene FUS
  • Oncogene TLS
  • POMP75
  • RNA-binding protein FUS
  • TLShnRNP-P2
  • Translocated in liposarcoma protein

Background

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Involved in placental, cardiac, hepatic and embryonic development. NCOA6 is over-expressed in several breast cancer cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FUS Antibody (NB100-2599)(3)

Review for FUS Antibody (NB100-2599) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-2599:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ICC Mouse 04/01/2017
View

Summary

ApplicationImmunocytochemistry
Sample Tested brain and spinal cord
SpeciesMouse
LotN/A

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FUS Antibody (NB100-2599) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional FUS Products

Bioinformatics Tool for FUS Antibody (NB100-2599)

Discover related pathways, diseases and genes to FUS Antibody (NB100-2599). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FUS Antibody (NB100-2599)

Discover more about diseases related to FUS Antibody (NB100-2599).
 

Pathways for FUS Antibody (NB100-2599)

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PTMs for FUS Antibody (NB100-2599)

Learn more about PTMs related to FUS Antibody (NB100-2599).

Blogs on FUS.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

Amyotrophic Lateral Sclerosis Infographic
Amyotrophic lateral sclerosis is a neurological disease which impacts motor neurons that are involved in muscle movement throughout the body. The progressive degeneration of neurons causes weakened muscles and can lead to paralysis. There is no cure f...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
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1
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Anonymous
04/01/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol FUS
Entrez
Uniprot