Fumarase Antibody

Images

 
Western Blot: Fumarase Antibody [NBP1-32893] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody ...read more
Immunocytochemistry/ Immunofluorescence: Fumarase Antibody [NBP1-32893] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-32893] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:500 dilution.
Western Blot: Fumarase Antibody [NBP1-32893] - Mouse liver lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: Fumarase Antibody [NBP1-32893] - Rat liver lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:5000.
Western Blot: Fumarase Antibody [NBP1-32893] - Sample (30 ug of whole cell lysate) A: 293T B: A431 10% SDS PAGE; antibody diluted at 1:5000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
     

Genetic Strategies

   

Order Details

Fumarase Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Fumarate hydratase. The exact sequence is proprietary.
Localization
Mitochondrion, Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Fumarase Antibody

  • EC 4.2.1.2
  • fumarase
  • fumarate hydratase
  • fumarate hydratase, mitochondrial
  • HLRCC
  • LRCC
  • MCL
  • MCUL1

Background

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol FH