Fumarase Antibody (OTI1F10) - Azide and BSA Free

Western Blot: Fumarase Antibody (OTI1F10) - Azide and BSA Free [NBP2-70733] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fumarase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Fumarase.

Immunocytochemistry/Immunofluorescence: Fumarase Antibody (OTI1F10) - Azide and BSA Free [NBP2-70733] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Fumarase.

Immunohistochemistry: Fumarase Antibody (OTI1F10) - Azide and BSA Free [NBP2-70733] - Staining of paraffin-embedded Human endometrium tissue using anti-Fumarase mouse monoclonal antibody.

Flow Cytometry: Fumarase Antibody (OTI1F10) - Azide and BSA Free [NBP2-70733] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Fumarase antibody, and then analyzed by flow cytometry.

Western Blot: Fumarase Antibody (OTI1F10) - Azide and BSA Free [NBP2-70733] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: Fumarase Antibody (OTI1F10) - Azide and BSA Free [NBP2-70733] - Fumarase Antibody (1F10) [NBP1-47754

Immunohistochemistry: Fumarase Antibody (OTI1F10) - Azide and BSA Free [NBP2-70733] - Staining of paraffin-embedded Human Kidney tissue using anti-Fumarase mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1F10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Fumarase Antibody (OTI1F10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Fumarase Antibody (OTI1F10) - Azide and BSA Free (NBP1-47754) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human FH (NP_000134) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Theoretical MW: 50.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Fumarase Antibody (OTI1F10) - Azide and BSA Free

• EC 4.2.1.2
• fumarase
• fumarate hydratase
• fumarate hydratase, mitochondrial
• HLRCC
• LRCC
• MCL
• MCUL1

Background

Fumarase is encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FH