Fumarase Antibody (OTI1F10)

Images

 
Western Blot: Fumarase Antibody (1F10) [NBP1-47754] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunocytochemistry/ Immunofluorescence: Fumarase Antibody (1F10) [NBP1-47754] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Fumarase.
Immunohistochemistry: Fumarase Antibody (OTI1F10) [NBP1-47754] - Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human colon tissue using anti-Fumarase mouse monoclonal antibody.
Flow Cytometry: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Fumarase antibody, and then ...read more
Western Blot: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fumarase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human endometrium tissue using anti-Fumarase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human Kidney tissue using anti-Fumarase mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI1F10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Fumarase Antibody (OTI1F10) Summary

Immunogen
Full length human recombinant protein of human FH (NP_000134) produced in HEK293T cell.
Specificity
This antibody is specific for Homo sapiens fumarate hydratase (FH), nuclear gene encoding mitochondrial protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
Theoretical MW
50.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-47754 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Fumarase Antibody (OTI1F10)

  • EC 4.2.1.2
  • fumarase
  • fumarate hydratase
  • fumarate hydratase, mitochondrial
  • HLRCC
  • LRCC
  • MCL
  • MCUL1

Background

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form,differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion,where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-P

Publications for Fumarase Antibody (NBP1-47754)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fumarase Antibody (NBP1-47754). (Showing 1 - 1 of 1 FAQ).

  1. I'm going to be using this antibody for Immunohistochemistry. Can I use a secondary antibody directly conjugated with the HRP, without the biotin/avidin system? Would the sensitivity be drastically affected? The PBS working concentration is 1X, right (PBS 1X + 0.1% Triton X-100, pH 7.4)? Which will be the same used for primary antibody? Which is the most adequate HIER protocol for this antibody? Can you provide a list of references where they show satisfactory results using this antibody?
    • You may use NBP1-47754 with a secondary that is directly conjugated to HRP, but the sensitivity will be reduced. Using a biotin/strepavidin system allows greater staining essentially because multiple enzymes associate with each primary antibody. If you already have an HRP-conjugated secondary, I would start with that but keep in mind that you may need to change your detection system if you get no signal or weak staining. I'm sorry but we don't have any peer-reviewed references for this product. Here is the recommended protocol for antigen retrieval: Antigen Retrieval Solution: 0.01M Sodium Citrate Buffer, pH 6.0 To prepare stock solutions: Solution A. 0.1 M citric acid solution: dissolve 21.0 g of citric acid, monohydrate (C6H8O7.H2O) in 100 ml of dH2O. Solution B. 0.1M sodium citrate solution: dissolve 29.4 g trisodium citrate dihydrate (C6H5Na3O7.2H2O) in 100 ml of dH2O. Working solution: Add 9 ml of Stock solution A and 41 ml of stock solution B to 450 ml of dH2O. Adjust pH to 6.0.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70733AF350
Alexa Fluor 405 NBP2-70733AF405
Alexa Fluor 488 NBP2-70733AF488
Alexa Fluor 532 NBP2-70733AF532
Alexa Fluor 594 NBP2-70733AF594
Alexa Fluor 647 NBP2-70733AF647
Alexa Fluor 700 NBP2-70733AF700
Alexa Fluor 750 NBP2-70733AF750
Allophycocyanin NBP2-70733APC
Biotin NBP2-70733B
DyLight 350 NBP2-70733UV
DyLight 405 NBP2-70733V
DyLight 488 NBP2-70733G
DyLight 550 NBP2-70733R
DyLight 594 NBP2-70733DL594
DyLight 650 NBP2-70733C
DyLight 680 NBP2-70733FR
DyLight 755 NBP2-70733IR
FITC NBP2-70733F
HRP NBP2-70733H
Janelia Fluor 549 NBP2-70733JF549
Janelia Fluor 646 NBP2-70733JF646
PE NBP2-70733PE
PerCP NBP2-70733PCP

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Bioinformatics

Gene Symbol FH
Entrez
Uniprot