FoxD3 Antibody (5G9)

Images

 
Western Blot: FoxD3 Antibody (5G9) [NBP1-51685] - Analysis using FOXD3 mouse mAb against NTERA-2 (1), HUVE-12 (2), HEK293 (3), Hela (4), Jurkat (5), K562 (6), RAW264.7 (7), NIH/3T3 (8), and COS7 (9) cell lysate.
Immunohistochemistry-Paraffin: FoxD3 Antibody (5G9) [NBP1-51685] - Analysis of paraffin-embedded lung cancer tissues (left) and ovarian cancer tissues (right) using FOXD3 mouse mAb with DAB staining.

Product Details

Summary
Reactivity Hu, Mu, MkSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
5G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

FoxD3 Antibody (5G9) Summary

Immunogen
Purified recombinant fragment of human FOXD3 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FOXD3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for FoxD3 Antibody (5G9)

  • AIS1
  • CWH3
  • forkhead box D3
  • forkhead box protein D3
  • FoxD3
  • Genesis
  • HFH2
  • HNF3/FH transcription factor genesis

Background

The FOXD3 polyclonal antibody recognizes human FOXD3 also known as Forkhead box protein D3 and HNF3/FH transcription factor genesis. FOXD3 belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain, predicted molecular weight 48 kD. FOXD3 binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. FOXD3 is essential for multiple developmental processes in the vertebrate embryo, including neural crest development and maintenance of mammalian stem cell lineages. The poly6317 antibody has been shown to be useful for Western blotting.[ ][[[]] ][[[]] ][[[]] ][[[]] ][[[]] ][[[]] ][[[]] ][[[]] ][[[]] ][[[]] ][[[]] ][[[]] [][]] Oct4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Blogs on FoxD3.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

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Bioinformatics

Gene Symbol FOXD3
Entrez