FoxD3 Antibody (5G9)

Western Blot: FoxD3 Antibody (5G9) [NBP1-51685] - Analysis using FOXD3 mouse mAb against NTERA-2 (1), HUVE-12 (2), HEK293 (3), Hela (4), Jurkat (5), K562 (6), RAW264.7 (7), NIH/3T3 (8), and COS7 (9) cell lysate.

Immunohistochemistry-Paraffin: FoxD3 Antibody (5G9) [NBP1-51685] - Analysis of paraffin-embedded lung cancer tissues (left) and ovarian cancer tissues (right) using FOXD3 mouse mAb with DAB staining.

• Reactivity: Hu, Mu, Pm
• Applications: WB, ELISA, IHC
• Clone: 5G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FoxD3 Antibody (5G9) Summary

• Description: Novus Biologicals Mouse FoxD3 Antibody (5G9) (NBP1-51685) is a monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human FoxD3 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOXD3
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Ascites

Alternate Names for FoxD3 Antibody (5G9)

• AIS1
• CWH3
• forkhead box D3
• forkhead box protein D3
• FoxD3
• Genesis
• HFH2
• HNF3/FH transcription factor genesis

Background

The FOXD3 polyclonal antibody recognizes human FOXD3 also known as Forkhead box protein D3 and HNF3/FH transcription factor genesis. FOXD3 belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain, predicted molecular weight 48 kD. FOXD3 binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. FOXD3 is essential for multiple developmental processes in the vertebrate embryo, including neural crest development and maintenance of mammalian stem cell lineages. The poly6317 antibody has been shown to be useful for Western blotting.[ ][[[ ][[[ ][[[ ][[[ ][[[ ][[[ ][[[ ][[[ ][[[ ][[[ ][[[ ][[[ [][ Oct4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOXD3