Follistatin-related Gene Protein/FLRG/Fstl3 Antibody [Biotin]

• Reactivity: Hu
• Applications: ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Follistatin-related Gene Protein/FLRG/Fstl3 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Follistatin-related Gene Protein/FLRG
  Met27-Val263
  Accession # O95633
• Specificity: Detects human Follistatin-related Gene Protein/FLRG in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross‑reactivity with recombinant mouse FLRG is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FSTL3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture:Human Follistatin-related Gene Protein/FLRG Antibody (Catalog # MAB1288)
  ELISA Detection: Human Follistatin-related Gene Protein/FLRG Biotinylated Antibody (Catalog # BAF1288)
  Standard: Recombinant Human Follistatin-related Gene Protein/FLRG (Catalog # 1288-F3)TM)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Follistatin-related Gene Protein/FLRG/Fstl3 Antibody [Biotin]

• FLRG
• FLRGFSRP
• follistatin-like 3 (secreted glycoprotein)
• Follistatin-like 3
• Follistatin-like protein 3
• Follistatin-related gene protein
• follistatin-related protein 3
• FSTL3

Background

Follistatin-related gene protein (FLRG), also known as follistatin-like 3 (FSTL3) is a glycoprotein belonging to the follistatin-module protein family. Human FLRG cDNA encodes a 263 amino acid (aa) residue protein with a putative 26 aa signal peptide, an N-terminal domain, two cysteine-rich follistatin-like domains (FS) and a C‑terminal acidic domain. Compared to follistatin, FLRG lacks the third FS domain found in follistatin. In addition, FLRG also lacks the heparin-binding domain found within the first amino-terminal FS domain of follistatin. Mouse and human FLRG share approximately 83% aa sequence homology. Like follistatin, FLRG has been shown to bind and inhibit the activities of TGF-beta family ligands including activin, BMP-2, -6, -7 and GDF-8/myostatin. While both FLRG and follistatin are located in a wide and overlapping range of adult and fetal tissue, their sites of peak expression differ: FLRG most highly in heart, lung, kidney, placenta and testis, while follistatin is highest in ovary and pituitary. The expression of FLRG is upregulated by TGF-beta  and activin signaling through Smad proteins. Although FLRG is a secreted protein in many cell types, it has also been localized to the nuclear compartment in HeLa, 293 and CHO cells (1‑5).

1. Tsuchida, K. et al. (2000) J. Biol. Chem. 275:40778.
2. Sidis, Y. et al. (2002) Endocrinology 143:1613.
3. Tortoriello, D.V. et al. (2001) Endocrinology 142:3426.
4. Hill, J. et al. (2002) J. Biol. Chem. 277:40735.
5. Bartholin, L. et al. (2001) Oncogene 20:5409.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Follistatin-related Gene Protein/FLRG/Fstl3 Antibody (BAF1288)(3)

Publications using BAF1288

• Marissa J Schafer Quantification of GDF11 and Myostatin in Human Aging and Cardiovascular Disease Cell Metab, 2016-06-14;23(6):1207-15. 6/14/2016 [PMID: 27304512]
• H. Robert Bergen, Joshua N. Farr, Patrick M. Vanderboom, Elizabeth J. Atkinson, Thomas A. White, Ravinder J. Singh et al. Myostatin as a mediator of sarcopenia versus homeostatic regulator of muscle mass: insights using a new mass spectrometry-based assay Skeletal Muscle 2015-12-01 [PMID: 26180626]
• Miron P, Lambert J, Marcil A, Cowans NJ, Stamatopoulou A, Spencer K Maternal plasma levels of follistatin-related gene protein in the first trimester of pregnancies with Down syndrome. Prenat. Diagn., 2010-03-01;30(3):224-8. 2010-03-01 [PMID: 20063262] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: FSTL3
• Uniprot:
  • O95633()