Follistatin-related Gene Protein/FLRG/Fstl3 Antibody [Unconjugated]

Recombinant Human Follistatin-related Gene Protein/FLRG (Catalog # 1288-F3) inhibits Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC) induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase activity. Inhibition of Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) activity elicited by Recombinant Human Follistatin‑related Gene Protein/FLRG (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Follistatin-related Gene Protein/FLRG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1288). The ND50 is typically 1‑4 µg/mL.

• Reactivity: Hu
• Applications: ELISA, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Follistatin-related Gene Protein/FLRG/Fstl3 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human FLRG
  Met27-Val263
  Accession # O95633
• Specificity: Detects Follistatin-related Gene Protein/FLRG in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross‑reactivity with recombinant mouse FLRG is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FSTL3
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA 0.1-0.4 ug/mL
  • Neutralization 1-4 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Follistatin-related Gene Protein/FLRG/Fstl3 Antibody [Unconjugated]

• FLRG
• FLRGFSRP
• follistatin-like 3 (secreted glycoprotein)
• Follistatin-like 3
• Follistatin-like protein 3
• Follistatin-related gene protein
• follistatin-related protein 3
• FSTL3

Background

Follistatin-related gene protein (FLRG), also known as follistatin-like 3 (FSTL3) is a glycoprotein belonging to the follistatin-module protein family. Human FLRG cDNA encodes a 263 amino acid (aa) residue protein with a putative 26 aa signal peptide, an N-terminal domain, two cysteine-rich follistatin-like domains (FS) and a C‑terminal acidic domain. Compared to follistatin, FLRG lacks the third FS domain found in follistatin. In addition, FLRG also lacks the heparin-binding domain found within the first amino-terminal FS domain of follistatin. Mouse and human FLRG share approximately 83% aa sequence homology. Like follistatin, FLRG has been shown to bind and inhibit the activities of TGF-beta family ligands including activin, BMP-2, -6, -7 and GDF-8/myostatin. While both FLRG and follistatin are located in a wide and overlapping range of adult and fetal tissue, their sites of peak expression differ: FLRG most highly in heart, lung, kidney, placenta and testis, while follistatin is highest in ovary and pituitary. The expression of FLRG is upregulated by TGF-beta  and activin signaling through Smad proteins. Although FLRG is a secreted protein in many cell types, it has also been localized to the nuclear compartment in HeLa, 293 and CHO cells (1‑5).

1. Tsuchida, K. et al. (2000) J. Biol. Chem. 275:40778.
2. Sidis, Y. et al. (2002) Endocrinology 143:1613.
3. Tortoriello, D.V. et al. (2001) Endocrinology 142:3426.
4. Hill, J. et al. (2002) J. Biol. Chem. 277:40735.
5. Bartholin, L. et al. (2001) Oncogene 20:5409.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Follistatin-related Gene Protein/FLRG/Fstl3 Antibody (AF1288)(2)

Publications using AF1288

• Xiaoya Li, Hongwei Zhang, Xiaojing Ma, Yufei Wang, Xiaodong Han, Ying Yang et al. FSTL3 is highly expressed in adipose tissue of individuals with overweight or obesity and is associated with inflammation Obesity (Silver Spring) 1/1/2023 [PMID: 36502285]
• Nanda S, Savvidou M, Syngelaki A, Akolekar R, Nicolaides KH Prediction of gestational diabetes mellitus by maternal factors and biomarkers at 11 to 13 weeks. Prenat. Diagn., 2010-12-28;31(2):135-41. 2010-12-28 [PMID: 21268030] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: FSTL3
• Uniprot:
  • O95633()