GASP-1/WFIKKN2 Antibody [Unconjugated]

Images

 
There are currently no images for GASP-1/WFIKKN2 Antibody (AF2070).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

GASP-1/WFIKKN2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human GASP‑1/WFIKKN2
Leu35-His576
Accession # Q8TEU8
Specificity
Detects human GASP‑1/WFIKKN2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human GASP-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
WFIKKN2
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Neutralization 18.9 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF2070 in the following applications:

Publications
Read Publication using
AF2070 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GASP-1/WFIKKN2 Antibody [Unconjugated]

  • GASP1
  • GASP-1
  • Growth and differentiation factor-associated serum protein 1
  • hGASP-1
  • WAP, follistatin, immunoglobulin, kunitz and NTR domain-containing-relatedprotein
  • WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2
  • WAP, FS, Ig, two KU and NTR module related protein
  • WAP, kazal, immunoglobulin, kunitz and NTR domain-containing protein 2
  • WFIKKN2
  • WFIKKN-related protein
  • WFIKKNRP
  • WFIKKNRPmultivalent protease inhibitor protein

Background

Growth and differentiation factor-associated serum protein-1 (GASP-1) was isolated in a screen to identify proteins in mice that copurify with myostatin (GDF-8), a potent negative regulator of skeletal muscle mass (1). The GASP-1 cDNA encodes a 571 amino acid protein that contains a 29 amino acid signal sequence and is comprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin, two tandem Kunitz, and netrin (1). Two related human proteins which contain the same domain structure are called WFIKKN (based on the presence and order of these conserved domains) and WFIKKNRP (WFIKKN-related protein) (2). Mouse GASP-1 is homologous to human WFIKKNRP and mouse GASP-2 to human WFIKKN. Human GASP-1 is the same protein as WFIKKNRP.

WAP, follistatin, Kazal and netrin domains are all implicated in protease inhibition, and these proteins may be multivalent protease inhibitors (3). GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP-1 expression is highest in the brain, skeletal muscle, thymus and kidney while GASP-2 is abundant in the lung, skeletal muscle and liver (4). In the adult, GASP-1 is primarily expressed in the ovary, testis, and brain while GASP‑2 is in the pancreas, liver, and thymus (4). GASP-1 inhibits myostatin and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro (1). In addition, GASP-1 binds directly but independently to both mature myostatin and the myostatin propeptide (1). By amino acid sequence, human GASP-1 is 90% and 55% identical to mouse GASP-1 and human GASP-2, respectively.

  1. Hill, J.J. et al. (2003) Mol. Endo. 17:1144.
  2. Trexler, M. et al. (2001) Proc. Natl. Acad. Sci. 98:3705.
  3. Nagy, A. et al. (2003) Eur. Jour. Biochem. 270:2101.
  4. Trexler, M. et al. (2002) Biol. Chem. 383:223.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

788-G8/CF
Species: Hu, Mu, Rt
Applications: BA
DFN00
Species: Hu
Applications: ELISA
NBP2-81951
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NLS418
Species: Hu
Applications: IHC, IHC-P
NLS2756
Species: Hu, Pm
Applications: ICC, IHC, IHC-P
MAB8458
Species: Hu
Applications: CyTOF-ready, Flow, ICC
NLS1017
Species: Bt, Bv, Ca, Eq, Hu, Mu
Applications: IHC, IHC-P
MAB194
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, WB
NLS2576
Species: Hu, Pm, Rt
Applications: IHC, IHC-P
MAB21363
Species: Hu
Applications: IHC, IP, WB
NLS32
Species: Bt, Bv, Ca, Eq, Hu, Pm, Mu, Po, Pm, Rt
Applications: ICC/IF, IHC, IHC-P
DFLRG0
Species: Hu
Applications: ELISA
1958-GD/CF
Species: Hu, Mu, Rt
Applications: BA
AF2070
Species: Hu
Applications: WB, IP, B/N

Publications for GASP-1/WFIKKN2 Antibody (AF2070)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Review for GASP-1/WFIKKN2 Antibody (AF2070) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF2070:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous -
WB Mouse 01/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22868230
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GASP-1/WFIKKN2 Antibody (AF2070) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional GASP-1/WFIKKN2 Products

Bioinformatics Tool for GASP-1/WFIKKN2 Antibody (AF2070)

Discover related pathways, diseases and genes to GASP-1/WFIKKN2 Antibody (AF2070). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on GASP-1/WFIKKN2

There are no specific blogs for GASP-1/WFIKKN2, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Anonymous -
01/06/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol WFIKKN2
Uniprot