GASP-1/WFIKKN2 Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, IP, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

GASP-1/WFIKKN2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human GASP‑1/WFIKKN2
  Leu35-His576
  Accession # Q8TEU8
• Specificity: Detects human GASP‑1/WFIKKN2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human GASP-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: WFIKKN2
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Neutralization 18.9 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GASP-1/WFIKKN2 Antibody [Unconjugated]

• GASP1
• GASP-1
• Growth and differentiation factor-associated serum protein 1
• hGASP-1
• WAP, follistatin, immunoglobulin, kunitz and NTR domain-containing-relatedprotein
• WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2
• WAP, FS, Ig, two KU and NTR module related protein
• WAP, kazal, immunoglobulin, kunitz and NTR domain-containing protein 2
• WFIKKN2
• WFIKKN-related protein
• WFIKKNRP
• WFIKKNRPmultivalent protease inhibitor protein

Background

Growth and differentiation factor-associated serum protein-1 (GASP-1) was isolated in a screen to identify proteins in mice that copurify with myostatin (GDF-8), a potent negative regulator of skeletal muscle mass (1). The GASP-1 cDNA encodes a 571 amino acid protein that contains a 29 amino acid signal sequence and is comprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin, two tandem Kunitz, and netrin (1). Two related human proteins which contain the same domain structure are called WFIKKN (based on the presence and order of these conserved domains) and WFIKKNRP (WFIKKN-related protein) (2). Mouse GASP-1 is homologous to human WFIKKNRP and mouse GASP-2 to human WFIKKN. Human GASP-1 is the same protein as WFIKKNRP.

WAP, follistatin, Kazal and netrin domains are all implicated in protease inhibition, and these proteins may be multivalent protease inhibitors (3). GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP-1 expression is highest in the brain, skeletal muscle, thymus and kidney while GASP-2 is abundant in the lung, skeletal muscle and liver (4). In the adult, GASP-1 is primarily expressed in the ovary, testis, and brain while GASP‑2 is in the pancreas, liver, and thymus (4). GASP-1 inhibits myostatin and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro (1). In addition, GASP-1 binds directly but independently to both mature myostatin and the myostatin propeptide (1). By amino acid sequence, human GASP-1 is 90% and 55% identical to mouse GASP-1 and human GASP-2, respectively.

1. Hill, J.J. et al. (2003) Mol. Endo. 17:1144.
2. Trexler, M. et al. (2001) Proc. Natl. Acad. Sci. 98:3705.
3. Nagy, A. et al. (2003) Eur. Jour. Biochem. 270:2101.
4. Trexler, M. et al. (2002) Biol. Chem. 383:223.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GASP-1/WFIKKN2 Antibody (AF2070)(3)

Publications using AF2070

• L Périè, A Parenté, F Baraige, L Magnol, V Blanquet Alterations in Adiposity and Glucose Homeostasis in Adult Gasp-1 Overexpressing Mice Cell. Physiol. Biochem., 2017-12-08;44(5):1896-1911. 2017-12-08 [PMID: 29224025] (Western Blot, Mouse)
• Monestier O, Brun C, Heu K et al. Ubiquitous Gasp1 overexpression in mice leads mainly to a hypermuscular phenotype. BMC Genomics 10/10/2012 [PMID: 23046573]
• Caroline Brun, Olivier Monestier, Sébastien Legardinier, Abderrahman Maftah, Véronique Blanquet Murine GASP-1 N-glycosylation is not essential for its activity on C2C12 myogenic cells but alters its secretion Cellular Physiology and Biochemistry 2012-07-13 [PMID: 22868230]

Review for GASP-1/WFIKKN2 Antibody (AF2070) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 01/06/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22868230
• Species: Mouse

Bioinformatics

• Gene Symbol: WFIKKN2
• Uniprot:
  • Q8TEU8()