Follistatin-like 1/FSTL1 Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Follistatin-like 1/FSTL1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Follistatin-like 1/FSTL1
  Glu19-Ile306
  Accession # Q62356
• Specificity: Detects mouse Follistatin-like 1/FSTL1 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human Follistatin-like 1/FSTL1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FSTL1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Follistatin-like 1/FSTL1 Antibody [Unconjugated]

• Flik
• FLJ50214
• Follistatin-like 1
• Follistatin-like protein 1
• follistatin-related protein 1
• Follistatin-related Protein
• FRP
• FRPFLJ52277
• FSL1
• FSTL1
• TSC-36

Background

Mouse FSTL1, also known as FSL1 and follistatin-related protein (FRP), is a secreted protein that contains a follistatin-like domain, a Kazal-like domain and a von Willebrand factor C domain. Human FSTL1 shares 92% amino acid sequence identity with the mouse FSTL1, also known as TSC-36.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Follistatin-like 1/FSTL1 Antibody (AF1738)(12)

Publications using AF1738

• Y Zhang, Y Wang, G Zheng, Y Liu, J Li, H Huang, C Xu, Y Zeng, X Zhang, J Qin, C Dai, HO Hambrock, U Hartmann, B Feng, KK Mak, Y Liu, HY Lan, Y Huang, ZH Zheng, Y Xia Follistatin-like 1 (FSTL1) interacts with Wnt ligands and Frizzled receptors to enhance Wnt/beta-catenin signaling in obstructed kidneys in vivo The Journal of Biological Chemistry, 2022;0(0):102010. 2022-01-01 [PMID: 35525270] (IHC, Mouse)
• Z Huang, Z Zhang, Z Moazzami, R Heck, P Hu, H Nanda, K Ren, Z Sun, A Bartolomuc, Y Gao, D Chung, W Zhu, S Shen, HB Ruan Brown adipose tissue involution associated with progressive restriction in progenitor competence Cell Reports, 2022;39(2):110575. 2022-01-01 [PMID: 35417710] (Immunoprecipitation, Mouse)
• D Fang, X Shi, X Jia, C Yang, L Wang, B Du, T Lu, L Shan, Y Gao Ups and downs: the PPARgamma/p-PPARgamma seesaw of follistatin-like 1 and integrin receptor signaling in adipogenesis Molecular Metabolism, 2021;0(0):101400. 2021-01-01 [PMID: 34813964] (Western Blot, Mouse)
• D Feng, C Ngov, N Henley, N Boufaied, C Gerarduzzi Characterization of Matricellular Protein Expression Signatures in Mechanistically Diverse Mouse Models of Kidney Injury Sci Rep, 2019;9(1):16736. 2019-01-01 [PMID: 31723159] (Western Blot, Mouse)
• K Kretzschma, Y Post, M Bannier-Hé, A Mattiotti, J Drost, O Basak, VSW Li, M van den Bo, QD Gunst, D Versteeg, L Kooijman, S van der El, JH van Es, E van Rooij, MJB van den Ho, H Clevers Profiling proliferative cells and their progeny in damaged murine hearts Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018-01-01 [PMID: 30530645] (IHC-Fr, Mouse)
• S Yamada, N Itoh, T Nagai, T Nakai, D Ibi, A Nakajima, T Nabeshima, K Yamada Innate immune activation of astrocytes impairs neurodevelopment via upregulation of follistatin-like 1 and interferon-induced transmembrane protein 3 J Neuroinflammation, 2018;15(1):295. 2018-01-01 [PMID: 30348171] (Western Blot, Mouse)
• X Liu, Y Liu, X Li, J Zhao, Y Geng, W Ning Follistatin like-1 (Fstl1) is required for the normal formation of lung airway and vascular smooth muscle at birth PLoS ONE, 2017;12(6):e0177899. 2017-01-01 [PMID: 28574994] (Western Blot, Mouse)
• Follistatin-like 1 promotes cardiac fibroblast activation and protects the heart from rupture EMBO Mol Med, 2016;0(0):. 2016-01-01 [PMID: 27234440] (Western Blot, Mouse)
• Chaly Y, Fu Y, Marinov A, Hostager B, Yan W, Campfield B, Kellum J, Bushnell D, Wang Y, Vockley J, Hirsch R Follistatin-like protein 1 enhances NLRP3 inflammasome-mediated IL-1beta secretion from monocytes and macrophages. Eur J Immunol, 2014;44(5):1467-79. 2014-01-01 [PMID: 24470197]
• Xu J, Qi X, Gong J Fstl1 antagonizes BMP signaling and regulates ureter development. PLoS ONE, 2012;7(4):e32554. 2012-01-01 [PMID: 22485132] (IHC, Mouse)
• Ippagunta SK, Malireddi RK, Shaw PJ The inflammasome adaptor ASC regulates the function of adaptive immune cells by controlling Dock2-mediated Rac activation and actin polymerization. Nat. Immunol., 2011;12(10):1010-6. 2011-01-01 [PMID: 21892172] (Western Blot, Mouse)
• Miyamae T, Marinov AD, Sowders D, Wilson DC, Devlin J, Boudreau R, Robbins P, Hirsch R Follistatin-like protein-1 is a novel proinflammatory molecule. J. Immunol., 2006;177(7):4758-62. 2006-01-01 [PMID: 16982916] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: FSTL1
• Uniprot:
  • Q62356()