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Flt-3/Flk-2/CD135 Antibody (113308)

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Flt‑3/Flk‑2 was detected in immersion fixed M1 mouse myeloid leukemia cell line using Rat Anti-Mouse Flt‑3/Flk‑2 Monoclonal Antibody (Catalog # MAB7681) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Flt‑3/Flk‑2 was detected in immersion fixed M1 mouse myeloid leukemia cell line using Rat Anti-Mouse Flt‑3/Flk‑2 Monoclonal Antibody (Catalog # MAB7681) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
113308
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB7681U-100UG
Alexa Fluor 405
FAB7681V-100UG
Alexa Fluor 488
FAB7681G-100UG
Alexa Fluor 594
FAB7681T-100UG
Alexa Fluor 647
FAB7681R-100UG
Alexa Fluor 700
FAB7681N-100UG
Alexa Fluor 750
FAB7681S-100UG
Biotin
BAM7681
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7681-SP
Carrier Free
FAB7681V-100UG
Carrier Free
FAB7681U-100UG
Carrier Free
FAB7681T-100UG
Carrier Free
FAB7681S-100UG
Carrier Free
FAB7681R-100UG
Carrier Free
FAB7681N-100UG
Carrier Free
FAB7681G-100UG
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Flt-3/Flk-2/CD135 Antibody (113308) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Flt‑3/Flk‑2
Asn28-Ser544 (predicted)
Accession # Q00342
Specificity
Detects mouse Flt‑3/Flk‑2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Flt-3 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
FLT3
Purity
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 3
using
MAB7681 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Flt-3/Flk-2/CD135 Antibody (113308)

  • CD135 antigen
  • CD135
  • EC 2.7.10
  • fetal liver kinase 2
  • FL cytokine receptor
  • FLK2
  • Flk-2
  • FLT3 receptor tyrosine kinase
  • Flt3
  • Flt-3
  • Fms-like tyrosine kinase 3
  • fms-related tyrosine kinase 3
  • growth factor receptor tyrosine kinase type III
  • Stem cell tyrosine kinase 1
  • STK-1
  • STK1EC 2.7.10.1
  • Tyrosine-protein kinase receptor FLT3

Background

The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1 (stem cell tyrosine kinase) and designated CD135, is a member of the class III subfamily of receptor tyrosine kinases. This familay includes KIT, the receptor for SCF, and C-FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Mouse Flt-3 cDNA encodes a 992 amino acid (aa) residue type I membrane protein with a 27 aa residue signal peptide, a 517 aa extracellular domain with 10 potential N-linked glycosylation sites, a 20 aa residue transmembrane domain and a 428 aa residue cytoplasmic domain. Mouse Flt-3 shares 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3 Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.

  1. Rosnet, O. et al. (1996) Acta. Haemato. 95:218.
  2. Drexler, H.G. (1996) Leukemia 10:588.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Flt-3/Flk-2/CD135 Antibody (MAB7681) (0)

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Review for Flt-3/Flk-2/CD135 Antibody (MAB7681) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB7681:
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Flt-3/Flk-2/CD135 MAB7681
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reviewed by:
Anonymous
 Mouse 10/21/2018
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Summary

Sample TestedBone marrow
SpeciesMouse
LotI don't have it any more

Comments

CommentsWhole Femur Bone Marrow Sections, Fixed-Frozen / Decalcified

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FAQs for Flt-3/Flk-2/CD135 Antibody (MAB7681) (0)

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Secondary Antibodies

 

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Other Available Formats

Alexa Fluor 350 FAB7681U
Alexa Fluor 405 FAB7681V
Alexa Fluor 488 FAB7681G
Alexa Fluor 594 FAB7681T
Alexa Fluor 647 FAB7681R
Alexa Fluor 700 FAB7681N
Alexa Fluor 750 FAB7681S
Biotin BAM7681
Learn about Custom Antibody Labeling

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Flt-3/Flk-2/CD135 MAB7681
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 Anonymous
10/21/2018
Application:
Species: Mouse
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Bioinformatics

Gene Symbol FLT3
Uniprot