FLIP Antibody

Images

 
Western Blot: FLIP Antibody [NBP1-77020] - Analysis of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: FLIP Antibody [NBP1-77020] - 3T3 cells with FLIP antibody at 5 ug/ml.
Western Blot: FLIP Antibody [NBP1-77020] - Western blot analysis of FLIP in NIH/3T3 whole cell lysate with FLIP antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

FLIP Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid peptide near the carboxy terminus of mouse FLIP. The immunogen is located within the last 50 amino acids of FLIP.
Specificity
FLIP has short form (FLIPS) and long form (FLIPL) and antibody recognizes the FLIPL only.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CFLAR
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
FLIP antibody can be used for the detection of FLIP by Western blot at 1:500 to 1:1000 dilution. 55 kDa band can be detected. FLIP has short form (FLIPS) and long form (FLIPL) and antibody recognizes the FLIPL only.
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
FLIP Peptide (NBP1-77020PEP)
Publications
Read Publications using NBP1-77020.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 15090542)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Ion exchange chromatography

Alternate Names for FLIP Antibody

  • CASHFADD-like antiapoptotic molecule 1
  • CASP8 and FADD-like apoptosis regulator
  • CASP8AP1
  • Caspase homolog
  • Caspase-eight-related protein
  • Caspase-like apoptosis regulatory protein
  • caspase-related inducer of apoptosis
  • CASPER
  • CasperFLAME1
  • Cellular FLICE-like inhibitory protein
  • CFLAR
  • c-FLIPR
  • c-FLIPS
  • CLARPMACH-related inducer of toxicity
  • FADD-like apoptotic molecule
  • FLAME
  • FLAME-1
  • FLIP
  • I-FLICE
  • I-FLICEMRITc-FLIPFLIPc-FLIPL
  • Inhibitor of FLICE
  • usurpin beta
  • Usurpin

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family.Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and MRIT. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FLIP Antibody (NBP1-77020)(9)


Showing Publications 1 - 9 of 9.
Publications using NBP1-77020 Applications Species
Kim JH, Gupta SC, Park B et al. Turmeric (Curcuma longa) inhibits inflammatory nuclear factor (NF)-kB and NF-kB-regulated gene products and induces death receptors leading to suppressed proliferation, induced chemosensitization, and suppressed osteoclastogenesis. Mol Nutr Food Res. 2012 Mar [PMID: 22147524]

Details:
1. SEAPorter Assay kit (10055k): Fig 2B, Analysis of the effects of tumeric on the inhibition of TNF-induced NF-kB activation in NF-kB SEAP stably transfected cells. 2. FLIP/I-FLiCE pAb (IMG-116-1/116-2): Fig 5C (WB), HCT-116 cells treated with tumeric.
Phromnoi K, Reuter S, Sung B et al. A novel pentamethoxyflavone down-regulates tumor cell survival and proliferative and angiogenic gene products through inhibition of IkB kinase activation and sensitizes tumor cells to apoptosis by cytokines and chemotherapeutic agents. Mol Pharmacol. 2011 Feb [PMID: 20930110]

Details:
KBM-5 cells: 1. FLIP (IMG-116-1, -2): WB (Fig 4A) 2. IKKb/IKK2 (IMG-129A): IP Kinase Assay (Fig 2D), IP/WB (Fig 2D). Note, the antibody IP'd an active kinase. The antibody co'IP'd IKKa. 3. IKKa (IMG-136a): WB (Figs 2C, 2D). Note Fig 2D was generated with an IP (IKKb, IMG-129A)/WB (IKKa, IMG-136a) assay. Note: The KBM-5 (human chronic myeloid leukemia) cells were incubated with PMF then treated with TNF-alpha, Figs 2C, 2D, 4A.
Aggarwal S, Ichikawa H, Takada Y et al. Curcumin (diferuloylmethane) down-regulates expression of cell proliferation and antiapoptotic and metastatic gene products through suppression of IkappaBalpha kinase and Akt activation. Mol Pharmacol. 2006 Jan [PMID: 16219905]
Ichikawa H, Takada Y, Murakami A, Aggarwal BB. Identification of a novel blocker of I kappa B alpha kinase that enhances cellular apoptosis and inhibits cellular invasion through suppression of NF-kappa B-regulated gene products. J Immunol. 2005 Jun 1 [PMID: 15905586]
Takada Y, Andreeff M, Aggarwal BB. Indole-3-carbinol suppresses NF-kappaB and IkappaBalpha kinase activation, causing inhibition of expression of NF-kappaB-regulated antiapoptotic and metastatic gene products and enhancement of apoptosis in myeloid and leukemia cells. Blood. 2005 Jul 15 [PMID: 15811958]
Takada Y, Fang X, Jamaluddin MS et al. Genetic deletion of glycogen synthase kinase-3beta abrogates activation of IkappaBalpha kinase, JNK, Akt, and p44/p42 MAPK but potentiates apoptosis induced by tumor necrosis factor. J Biol Chem. 2004 Sep 17 [PMID: 15252041]
Takada Y, Khuri FR, Aggarwal BB. Protein farnesyltransferase inhibitor (SCH 66336) abolishes NF-kappaB activation induced by various carcinogens and inflammatory stimuli leading to suppression of NF-kappaB-regulated gene expression and up-regulation of apoptosis. J Biol Chem. 2004 Jun 18 [PMID: 15090542]

Details:
Antibodies cited [WB]: 1. IKKa [IMG-136A] (Jurkat cells incubated with SCH 66336 activated with TNF), Fig. 2d (Jurkat cells treated with TNF), Fig. 2e IP (Jurkat cells incubated with SCH 66336 activated with TNF), Fig. 2d IP (Jurkat cells treated with TNF
Kishimoto H, Sprent J. A defect in central tolerance in NOD mice. Nat Immunol. 2001 Nov [PMID: 11668341]
Kim K, Fisher MJ, Xu SQ, el-Deiry WS. Molecular determinants of response to TRAIL in killing of normal and cancer cells. Clin Cancer Res. 2000 Feb [PMID: 10690508]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Blogs on FLIP.

TRAIL-R2: The Trail Less Traveled
Cells undergo apoptotic programmed cell death in response to various stimuli, and this key mechanism is necessary for cellular morphogenesis, tissue homeostasis, and host defense. Particular cytokines such as tumor necrosis factor (TNF) and the Fas li...  Read full blog post.

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Bioinformatics

Gene Symbol CFLAR
Entrez
Uniprot