FLIP Antibody


Western Blot: FLIP Antibody [NBP1-77020] - Analysis of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: FLIP Antibody [NBP1-77020] - 3T3 cells with FLIP antibody at 5 ug/ml.
Western Blot: FLIP Antibody [NBP1-77020] - Western blot analysis of FLIP in NIH/3T3 whole cell lysate with FLIP antibody at 1:500 dilution.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
1 mg/ml

Order Details

FLIP Antibody Summary

Antibody was raised against a peptide corresponding to amino acids 449 to 465 of mouse FLIPL/CASHa.
Ion exchange chromatography
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Ion exchange chromatography


Application Notes
FLIP antibody can be used for the detection of FLIP by Western blot at 1:500 to 1:1000 dilution. 55 kDa band can be detected. FLIP has short form (FLIPS) and long form (FLIPL) and antibody recognizes the FLIPL only.
Control Peptide
FLIP Peptide (NBP1-77020PEP)
Read Publications using NBP1-77020.

Reactivity Notes


Alternate Names for FLIP Antibody

  • CASHFADD-like antiapoptotic molecule 1
  • CASP8 and FADD-like apoptosis regulator
  • CASP8AP1
  • Caspase homolog
  • Caspase-eight-related protein
  • Caspase-like apoptosis regulatory protein
  • caspase-related inducer of apoptosis
  • CasperFLAME1
  • Cellular FLICE-like inhibitory protein
  • c-FLIPR
  • c-FLIPS
  • CLARPMACH-related inducer of toxicity
  • FADD-like apoptotic molecule
  • FLAME-1
  • Inhibitor of FLICE
  • usurpin beta
  • Usurpin


Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family.Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and MRIT. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FLIP Antibody (NBP1-77020)(9)

Showing Publications 1 - 9 of 9.
Publications using NBP1-77020 Applications Species
Kim JH, Gupta SC, Park B et al. Turmeric (Curcuma longa) inhibits inflammatory nuclear factor (NF)-kB and NF-kB-regulated gene products and induces death receptors leading to suppressed proliferation, induced chemosensitization, and suppressed osteoclastogenesis. Mol Nutr Food Res. 2012 Mar [PMID:22147524]
Phromnoi K, Reuter S, Sung B et al. A novel pentamethoxyflavone down-regulates tumor cell survival and proliferative and angiogenic gene products through inhibition of IkB kinase activation and sensitizes tumor cells to apoptosis by cytokines and chemotherapeutic agents. Mol Pharmacol. 2011 Feb [PMID:20930110]
Aggarwal S, Ichikawa H, Takada Y et al. Curcumin (diferuloylmethane) down-regulates expression of cell proliferation and antiapoptotic and metastatic gene products through suppression of IkappaBalpha kinase and Akt activation. Mol Pharmacol. 2006 Jan [PMID:16219905]
Ichikawa H, Takada Y, Murakami A, Aggarwal BB. Identification of a novel blocker of I kappa B alpha kinase that enhances cellular apoptosis and inhibits cellular invasion through suppression of NF-kappa B-regulated gene products. J Immunol. 2005 Jun 1 [PMID:15905586]
Takada Y, Andreeff M, Aggarwal BB. Indole-3-carbinol suppresses NF-kappaB and IkappaBalpha kinase activation, causing inhibition of expression of NF-kappaB-regulated antiapoptotic and metastatic gene products and enhancement of apoptosis in myeloid and leukemia cells. Blood. 2005 Jul 15 [PMID:15811958]
Takada Y, Fang X, Jamaluddin MS et al. Genetic deletion of glycogen synthase kinase-3beta abrogates activation of IkappaBalpha kinase, JNK, Akt, and p44/p42 MAPK but potentiates apoptosis induced by tumor necrosis factor. J Biol Chem. 2004 Sep 17 [PMID:15252041]
Takada Y, Khuri FR, Aggarwal BB. Protein farnesyltransferase inhibitor (SCH 66336) abolishes NF-kappaB activation induced by various carcinogens and inflammatory stimuli leading to suppression of NF-kappaB-regulated gene expression and up-regulation of apoptosis. J Biol Chem. 2004 Jun 18 [PMID:15090542]
Kishimoto H, Sprent J. A defect in central tolerance in NOD mice. Nat Immunol. 2001 Nov [PMID:11668341]
Kim K, Fisher MJ, Xu SQ, el-Deiry WS. Molecular determinants of response to TRAIL in killing of normal and cancer cells. Clin Cancer Res. 2000 Feb [PMID:10690508]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

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Research Areas for FLIP Antibody (NBP1-77020)

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Blogs on FLIP.

TRAIL-R2: The Trail Less Traveled
Cells undergo apoptotic programmed cell death in response to various stimuli, and this key mechanism is necessary for cellular morphogenesis, tissue homeostasis, and host defense. Particular cytokines such as tumor necrosis factor (TNF) and the Fas li...  Read full blog post.

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Product PDFs


Gene Symbol CFLAR

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