FLIP Antibody

Images

 
Western Blot: FLIP Antibody [NB100-56141] - analysis of FLIP in human ovarian cancer cell lines using anti-FLIP antibody. The primary antibody was used at a dilution of 1:200 and incubated for overnight at 4C in 5% milk ...read more
Immunohistochemistry: FLIP Antibody [NB100-56141] - Immunhohistochemical analysis of FLIP in tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using NB100-56141 at 1:2000. A-C, tissue ...read more
Immunohistochemistry: FLIP Antibody [NB100-56141] - Immunhohistochemical analysis of FLIP in two tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using NB100-56141 at 1:2000. A, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

FLIP Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 75-92 (DRKAVETHLLRNPHLVS) of human FLIP was used as immunogen, GenBank no. gi|12643547. This amino acid sequnce is 100% conserved in FLIP long and short, and various FLIP isoforms as denoted in the NCBI BLAST data base. http://www.ncbi.nlm.nih.gov/blast/.
Specificity
The gene encoding FLIP produces, by alternative splicing, 24 different transcripts, all with introns, putatively encoding 22 different protein isoforms. The molecular weights of these isoforms may vary. Therefore, the molecular weight of FLIP observed by western blot may vary according to the isoforms present in the sample.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CFLAR
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
WB: 1:1000-1:2000~; IHC (paraffin): 1:1000-1:5000~; IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents)~; IP: 1:50-1:200
Reviewed Applications
Read 1 Review rated 4
using
NB100-56141 in the following applications:

Reactivity Notes

Human Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for FLIP Antibody

  • CASHFADD-like antiapoptotic molecule 1
  • CASP8 and FADD-like apoptosis regulator
  • CASP8AP1
  • Caspase homolog
  • Caspase-eight-related protein
  • Caspase-like apoptosis regulatory protein
  • caspase-related inducer of apoptosis
  • CASPER
  • CasperFLAME1
  • Cellular FLICE-like inhibitory protein
  • CFLAR
  • c-FLIPR
  • c-FLIPS
  • CLARPMACH-related inducer of toxicity
  • FADD-like apoptotic molecule
  • FLAME
  • FLAME-1
  • FLIP
  • I-FLICE
  • I-FLICEMRITc-FLIPFLIPc-FLIPL
  • Inhibitor of FLICE
  • usurpin beta
  • Usurpin

Background

Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the caspase (ICE/CED-3) protease family. Most if not all, forms of apoptosis involve activation of the caspases. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the caspase family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by death receptors (reviewed in Peter, 2004). However, the functions of FLIP remain to be fully elucidated and there is some evidence FLIP may also act as an activator of caspase-8/-10 in certain cases, thereby taking on an apoptosis promoting role. FLIP has a short form and a long form. Additionally, four splice variants of c-FLIPs have been identified and termed FLIP a, b, g, and d. NB100-56141 recognizes FLIP. Human FLIP long is a 480 amino acid protein, GenBank no. gi|12643547. Human FLIP short is a 221 amino acid protein, GenBank no. AAC51623.1. NB100-56141 will recognize FLIP long and short, and FLIP isoforms that contain the peptide immunogen sequence (DRKAVETHLLRNPHLVSD), including FLIP a, b, g, and d.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for FLIP Antibody (NB100-56141) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-56141:
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Images Ratings Applications Species Date Details
Western Blot FLIP NB100-56141
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reviewed by:
Anonymous
WB Human 12/05/2014
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Summary

ApplicationWestern Blot
Sample TestedHuman ovarian cancer celll lines
SpeciesHuman

Blocking

Blocking Details5%milk in PBST 1h RT

Primary Anitbody

Dilution Ratio1:1200 O.N 4C in 5%milk in PBST

Secondary Antibody

Secondary DescriptionGoat anti rabbit
Secondary Manufacturer Cat#NB100-56141

Details

Detection NotesECL

Product General Protocols

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Video Protocols

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FAQs for FLIP Antibody (NB100-56141) (0)

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Blogs on FLIP.

TRAIL-R2: The Trail Less Traveled
Cells undergo apoptotic programmed cell death in response to various stimuli, and this key mechanism is necessary for cellular morphogenesis, tissue homeostasis, and host defense. Particular cytokines such as tumor necrosis factor (TNF) and the Fas li...  Read full blog post.

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Recent Reviews

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Anonymous
12/05/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol CFLAR
Entrez