FLASH Antibody - BSA Free

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Western Blot: FLASH Antibody [NB500-218] - Analysis of FLASH in NIH3T3 cells using this antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

FLASH Antibody - BSA Free Summary

Immunogen
N-terminus of the FLASH protein.
Localization
Cytoplasmic and Nuclear
Specificity
Reacts with the N terminus of the FLASH protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP8AP2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 -1:1000
Application Notes
Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
212 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse. Others species have not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FLASH Antibody - BSA Free

  • CASP8 associated protein 2
  • caspase 8 associated protein 2
  • CED-4
  • FLASHKIAA1315CASP8-associated protein 2
  • FLICE associated huge
  • FLICE-associated huge protein
  • FLJ11208
  • human FLASH
  • RIP25FLASH homolog RIP25

Background

Fas is a cell-surface receptor molecule that relays apoptotic signals into cells. When Fas is activated by the binding of its ligand, the proteolytic protein caspase-8 is recruited to a signaling complex known as DISC by binding to a Fas-associated adapter protein. A new protein, FLASH, contains a motif with oligomerizing activity whose sequence is similar to that of the Caenorhabditis elegans protein CED-4, and another domain (DRD domain) that interacts with a death-effector domain in caspase-8 or in the adapter protein. FLASH is likely a component of the DISC signaling complex and is necessary for the activation of caspase-8 in Fas-mediated apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for FLASH Antibody (NB500-218). (Showing 1 - 1 of 1 FAQ).

  1. Would you be able to confirm the immunogen and epitopes for NB500-218, NB500-219 - FLASH Antibodies?
    • NB500-219 was made to the C-terminal region of the FLASH protein and will recognize an epitope in this region. Product NB500-218 is made to the N-terminal region and will recognize an epitope in the N-terminal region.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CASP8AP2
Entrez