FKBP52/FKBP4 Antibody

Images

 
Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FKBP4 antibody.
Immunocytochemistry/ Immunofluorescence: FKBP52/FKBP4 Antibody [NBP1-33702] - Paraformaldehyde-fixed A431, using (NBP1-33702) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: FKBP52/FKBP4 Antibody [NBP1-33702] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - H1299 lysates using NBP1-33702 at a dilution of 1:5000. 30ug whole cell lysate loaded on 10% SDS PAGE gel.
Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - A. 30 ug GL261 whole cell lysate/extract, B. 30 ug C8D30 whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - 30 ug Rat 2 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - A. 30 ug 293T whole cell lysate/extract, B. 30 ug A431 whole cell lysate/extract, C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract E. ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.99 mg/ml
     

Genetic Strategies

   

Order Details

FKBP52/FKBP4 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human FKBP4. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FKBP4
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:10-1:500

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.99 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FKBP52/FKBP4 Antibody

  • 51 kDa FK506-binding protein
  • 52 kDa FKBP
  • 59 kDa immunophilin
  • EC 5.2.1.8
  • FK506 binding protein 4, 59kDa
  • FK506-binding protein 4 (59kD)
  • FK506-binding protein 4
  • FKBP4
  • FKBP-4
  • FKBP51
  • FKBP52
  • FKBP-52
  • FKBP52T-cell FK506-binding protein, 59kD
  • FKBP59
  • FKBP5952 kDa FK506-binding protein
  • HBI
  • HSP binding immunophilin
  • Hsp56
  • HSP-binding immunophilin
  • Immunophilin FKBP52
  • p52
  • p59
  • peptidyl-prolyl cis-trans isomerase FKBP4
  • peptidylprolyl cis-trans isomerase
  • PPIase FKBP4
  • PPIase
  • rotamase

Background

Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin Abinding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90 (4). Although FKBP52 and FKBP51 share approx. 75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90- binding characteristics (3, 5). Also, whereas FKBP51 typically has a role with the progesterone receptor, FKBP52 has been found to be linked to the progesterone, androgen and glucocorticoid receptors (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FKBP4
Entrez