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FIZ1 Antibody

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Western Blot: FIZ1 Antibody [NBP2-82793] - WB Suggested Anti-FLJ14768 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: Human Kidney
Western Blot: FIZ1 Antibody [NBP2-82793] - WB Suggested Anti-FLJ14768 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: Human Kidney
Immunohistochemistry-Paraffin: FIZ1 Antibody [NBP2-82793] - Rabbit Anti-FLJ14768 Antibody. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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FIZ1 Antibody Summary

Immunogen
The immunogen is a synthetic peptide directed towards the C terminal region of human FIZ1. Peptide sequence: HCGALYAALAALEEHRRVSHGEGGGEEAAAAAREREPASGEPPSGSGRGK The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Gene
FIZ1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1.0 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for FIZ1 Antibody

  • FLJ00416
  • FLT3-interacting zinc finger 1
  • flt3-interacting zinc finger protein 1
  • Zinc finger protein 798
  • ZNF798FLJ14768

Background

The FIZ1 gene encodes zinc finger protein, which interacts with a receptor tyrosine kinase involved in the regulation ofhematopoietic and lymphoid cells. This gene product also interacts with a transcription factor that regulates theexpression of rod-specific

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-80739
Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739] - WB analysis of lysates from (A) Human Embryonic Kidney 293 / HEK293 and (B) Human Embryonic carcinoma NCCIT cell lines using Flt-3/Flk-2/CD135 antibody (clone 7E8.2C8) at 2ug/ml concentration. The antibody detected single specific bands in both samples at the expected molecular weight position. Image from the standard format of this antibody.
Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739] - HEK293 cells were stained (surface) with Flt-3/Flk-2/CD135 antibody (clone: 7E8.2C8; red) or isotype control (mouse IgG2 lambda; blue) and positive staining observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis Image from the standard format of this antibody.
Immunocytochemistry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver cancer using Flt-3/Flk-2/CD135 antibody (clone 7E2.2C8) at 15 ug/ml concentration. The antibody generated specific cytoplasmic immunostaining of FLT3 in the cancerous cells w
Species: Hu
Applications: CyTOF-ready, Flow, ICC, IHC, IHC-P, WB
Rhodopsin Antibody
(4D2)
NBP2-59690
Immunohistochemistry-Frozen: Rhodopsin Antibody (4D2) [NBP2-59690] - Zebrafish retina (outer nuclear layer). PFA and methanol fixed 7 um sections. Rhodopsin antibody O/N incubation at 1:500. IHC-Fr image submitted by a verified customer review.
Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Bovine photoreceptor membranes showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Lane 1: MW ladder. Lane 2: 10 ug. Lane 3: 5 ug. Lane 4: 2.5 ug.. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000.
Immunohistochemistry: Rhodopsin Antibody (4D2) [NBP2-59690] - Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Tissue: retina. Species: Mouse. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Localization: Staining of photoreceptor outer segment (OS). Other layers of the retina: IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GCL ganglion cell layer..
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(3 Publications)
Species: Am, Av, Fi, Ma, Ze
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AF2945
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(4 Publications)
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H00001406-M02
Western Blot: CRX/CORD2 Antibody (4G11) [H00001406-M02] - Analysis of CRX expression in transfected 293T cell line by CRX monoclonal antibody (M02), clone 4G11.Lane 1: CRX transfected lysate(32 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: CRX/CORD2 Antibody (4G11) [H00001406-M02] - Retinal development is recapitulated in differentiating stem cells. Immunostaining of day 49 cultures shows remnants of retinal organization. CRX+ photoreceptor progenitors in green and mCherry+ RGCs in magenta. CRX+ cells - white arrows - appear to segregate from mCherry+ axons, suggesting division between the outer nuclear layer and the nerve fiber layer. Scale bar?=?500?um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep16595), licensed under a CC-BY licence.
ELISA: CRX/CORD2 Antibody (4G11) [H00001406-M02] - Detection limit for recombinant GST tagged CRX is approximately 0.03ng/ml as a capture antibody.
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Species: Bv, Fi, Hu, Mu
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
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Immunohistochemistry-Paraffin: RhoD Antibody [NBP1-58359] - Human Tonsil Tissue, 4-8ug/ml.
Western Blot: RhoD Antibody [NBP1-58359] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml
Immunohistochemistry: RhoD Antibody [NBP1-58359] - Human Bronchial Epithelial Tissue Observed Staining: Cytoplasm and membrane of bronchial epithelial tissue Primary Antibody Concentration: 1 : 600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X.
Species: Hu
Applications: IHC, IHC-P, WB
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Immunohistochemistry-Frozen: PDE6 beta Antibody [NB120-5663] - Specific labeling of the photoreceptor outer segments with minimal background. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: PDE6 beta Antibody [NB120-5663] - Mouse retina outer segments.
Western Blot: PDE6 beta Antibody [NB120-5663] - Analysis of PDE6 beta. Lane 1 demostrates no detection on extracts of RD mice. Lane 2 shows a single band at ~90 kDa on Balb/C extracts.
(1 Review)
(2 Publications)
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STAT5a/b Antibody
[Unconjugated] - Pan ...
AF2168
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse STAT5a/b Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2168) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for STAT5a at approximately 94 kDa and STAT5b at approximately 92 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for STAT5a/b at approximately 91 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human/Mouse STAT5a/b Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2168). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Glutathione S
Transferase kappa 1 A ...
NBP2-16686
Western Blot: Glutathione S Transferase kappa 1 Antibody [NBP2-16686] - Wild-type (WT) and Glutathione S Transferase kappa 1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glutathione S Transferase kappa 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Glutathione S Transferase kappa 1 Antibody [NBP2-16686] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP2-16686] - Immunohistochemical analysis of paraffin-embedded gastric ca, using antibody at 1:500 dilution.
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Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NPM1 Antibody -
BSA Free
NB110-61646
Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in OCI-AML3 cells.
Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in HL-60 cells (negative control).
Western Blot: Nucleophosmin Antibody [NB110-61646] - Detection of mutant Nucleophosmin in OCI-AML3 lysates.
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(3 Publications)
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Immunohistochemistry-Paraffin: SLC25A33 Antibody [NBP1-32499] - Paraffin-embedded SAOS-2 xenograft, using antibody at 1:500 dilution.
(1 Publication)
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IL-7 [Unconjugated]
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CD43/Sialophorin
Antibody (SPM503)
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Immunocytochemistry/Immunofluorescence: CD43/Sialophorin Antibody (SPM503) [NBP2-32822] - Immunofluorescence Analysis of K562 cells labeling CD43 with CD43/Sialophorin Antibody (SPM503) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is NucSpot Live 650.
Western Blot: CD43/Sialophorin Antibody (SPM503) [NBP2-32822] - Western Blot Analysis of K562 cell lysate using CD43/Sialophorin Antibody (SPM503).
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPM503) [NBP2-32822] - Formalin-fixed, paraffin-embedded human tonsil stained with CD43/Sialophorin Mouse Monoclonal Antibody (SPM503).
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SLCO6A1 Antibody
NBP2-49118
Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining in human testis and endometrium tissues using anti-SLCO6A1 antibody. Corresponding SLCO6A1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining of human testis shows high expression.
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Applications: IHC, IHC-P
STAT5b Antibody
[Unconjugated]
AF1584
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, M1 mouse myeloid leukemia cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse STAT5b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1584) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT5b at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for STAT5b at approximately 92 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse STAT5b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1584). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and STAT5b knockout HeLa cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse STAT5b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1584) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT5b at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockoutHeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Review)
(3 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, ICC, IP, ICFlow, KO, Simple Western, WB
ARF4L Antibody
NBP2-92876
Immunohistochemistry-Paraffin: ARF4L Antibody [NBP2-92876] - Paraffin-embedded human placenta using ARF4L .
Immunohistochemistry-Paraffin: ARF4L Antibody [NBP2-92876] - Paraffin-embedded mouse testis using ARF4L .
Western Blot: ARF4L Antibody [NBP2-92876] - Analysis of extracts of various cell lines, using ARF4L at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
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Applications: ICC/IF, IHC, IHC-P, WB
LIF [Unconjugated]
7734-LF
LIF [Unconjugated]
(24 Publications)
Species: Hu
Applications: BA
LPCAT1 Antibody
NBP1-88923
Western Blot: LPCAT1 Antibody [NBP1-88923] - Analysis in human cell lines PC-3 and Caco-2 using anti-LPCAT1 antibody. Corresponding LPCAT1 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Immunohistochemistry-Paraffin: LPCAT1 Antibody [NBP1-88923] - Staining of human cerebral cortex, kidney, liver and lung using Anti-LPCAT1 antibody NBP1-88923 (A) shows similar protein distribution across tissues to independent antibody NBP1-88922 (B).
Immunohistochemistry-Paraffin: LPCAT1 Antibody [NBP1-88923] - Analysis in human lung and liver tissues. Corresponding LPCAT1 RNA-seq data are presented for the same tissues.
(1 Review)
(2 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
c-Myc Antibody
(9E10) - BSA Free
NB600-302
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (9E10) NB600-302R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1003277) licensed under a CC-BY licence.
(4 Reviews)
(38 Publications)
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
VDR/NR1I1/Vitamin
D Receptor Antibody ( ...
PP-H4537-00
VDR/NR1I1/Vitamin D Receptor Antibody (H4537) [Unconjugated]
(2 Publications)
Species: Hu
Applications: DirELISA, IHC, IP, WB
FIZ1 Antibody
NBP2-82793
FIZ1 Antibody
Species: Hu
Applications: WB, IHC, IHC-P

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Bioinformatics

Gene Symbol FIZ1