Novus Biologicals products are now on bio-techne.com

CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin

Images

 
Western Blot: CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-human CD43 (MEM-59).Lane 1: ...read more
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Staining of human spleen (paraffin sections) using anti-CD43 (MEM-59).
Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Surface staining of human peripheral blood with anti-CD43 (MEM-59) PE.
Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Surface staining of human peripheral blood with anti-CD43 (MEM-59) APC-CyTM7.
Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Surface staining of human peripheral blood with anti-CD43 (MEM-59) biotin / streptavidin-APC.
Flow (Cell Surface): CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin [NBP2-62227] - Surface staining of human peripheral blood with anti-CD43 (MEM-59) FITC.
Staining of human spleen (paraffin sections) using anti-CD43 (MEM-59).Commercially tested by LifeSpan BioSciences.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Func, IHC, IP
Clone
MEM-59
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin Summary

Additional Information
Clone MEM-59 was used by HLDA to establish CD designation.
Immunogen
Human T lymphocytes.
Specificity
The antibody MEM-59 recognizes a neuraminidase-sensitive extracellular epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and erythrocytes.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPN
Purity
Protein A purified
Endotoxin Note
Endotoxin level is less than 0.01 EU/ug of the protein
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-5 ug/ml
  • Functional
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
Functional application: The antibody MEM-59 activates T lymphocytes and also induces apoptosis in hemopoietic progenitor cells. Immunohistochemistry (paraffin sections): Positive tissue: spleen, thymus, tonsil.
Theoretical MW
135 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4), 0.2 um filter sterilized
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin

  • CD43 antigen
  • CD43
  • CD43)
  • Galactoglycoprotein
  • GALGP
  • GPL115
  • Leukocyte sialoglycoprotein
  • Leukosialin
  • LSN
  • Ly-48
  • sialophorin (gpL115, leukosialin, CD43)
  • Sialophorin
  • SPN

Background

CD43 is a key glycoprotein of thymocytes and T lymphocytes, which affects the physicochemical properties of the T-cell surface and plays a role in lectin binding. This antibody contains an extended rodlike structure that may protrude above the glycocalyx of the cell, allowing accessibility of multiple glycan chains for binding. During T-cell activation CD43 is actively removed from the T-cell-antigen-presenting cell contact site, thus suggesting CD43's role in negative regulation of the adaptive immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

7268-CT
Species: Hu
Applications: BA
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
AF114
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, WB
NBP1-43435
Species: Hu, Mu
Applications: Flow, WB
MAB4841
Species: Mu
Applications: CompCytotox, CyTOF-ready, Flow, ICC, IHC, IP, Tstim
NBP2-67309
Species: Hu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
AF1730
Species: Hu
Applications: AdBlk, CyTOF-ready, Flow, ICC
NBP2-70360
Species: Hu
Applications: CyTOF-ready, Flow, IMC, ICC/IF, IHC, IHC-P, WB
MAB3595
Species: Hu
Applications: CyTOF-ready, Flow, Simple Western, WB
BBA10
Species: Hu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
NBP1-49045
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
NB110-89474
Species: Bv, Ce, ChHa, Hu, Pm, Mu, Rt, RM
Applications: Dual ISH-IHC, Flow-CS, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, ISH, Simple Western, Single-Cell Western, WB
AF796
Species: Mu
Applications: AdBlk, IHC, WB
AF629
Species: Hu
Applications: CyTOF-ready, Flow, InhibCellGro, WB
AF1126
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, IP, WB
AF4117
Species: Rt
Applications: IHC, WB
NBP2-46157
Species: Hu
Applications: Flow, IHC, IHC-P, WB

Publications for CD43/Sialophorin Antibody (NBP2-62227) (0)

There are no publications for CD43/Sialophorin Antibody (NBP2-62227).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CD43/Sialophorin Antibody (NBP2-62227) (0)

There are no reviews for CD43/Sialophorin Antibody (NBP2-62227). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD43/Sialophorin Antibody (NBP2-62227) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CD43/Sialophorin Products

Research Areas for CD43/Sialophorin Antibody (NBP2-62227)

Find related products by research area.

Blogs on CD43/Sialophorin

There are no specific blogs for CD43/Sialophorin, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CD43/Sialophorin Antibody (MEM-59) - Low Endotoxin and receive a gift card or discount.

Bioinformatics

Gene Symbol SPN