FIP1/RCP Antibody - BSA Free

Immunohistochemistry-Paraffin: FIP1/RCP Antibody [NBP2-20033] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RAB11FIP1 antibody [N1N2], N-term (NBP2-20033) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

FIP1/RCP Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit FIP1/RCP Antibody - BSA Free (NBP2-20033) is a polyclonal antibody validated for use in IHC and WB. Anti-FIP1/RCP Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human FIP1/RCP. The exact sequence is proprietary.
• Localization: Recycling endosome; Isoform 2: Cytoplasmic vesicle; phagosome membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAB11FIP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 137 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for FIP1/RCP Antibody - BSA Free

• DKFZp686E2214
• EC 3.2.1.28
• EC 6.1.1.7
• FLJ22524
• FLJ22622
• MGC78448
• NOEL1A
• RAB11 coupling protein
• RAB11 family interacting protein 1 (class I)
• rab11 family-interacting protein 1
• Rab11-FIP1
• Rab-coupling protein
• Rab-interacting recycling protein
• RCPRab effector protein

Background

Proteins of the large Rab GTPase family (see RAB1A; MIM 179508) have regulatory roles in the formation, targeting, and fusion of intracellular transport vesicles. RAB11FIP1 is one of many proteins that interact with and regulate Rab GTPases (Hales et al., 2001 [PubMed 11495908]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for FIP1/RCP Antibody (NBP2-20033)(2)

Publications using NBP2-20033

• Watson J, Ferguson HR, Brady RM et al. Spatially resolved phosphoproteomics reveals fibroblast growth factor receptor recycling-driven regulation of autophagy and survival Nature communications 2022-11-03 [PMID: 36329028] (WB, Human)
• Smith MP, Ferguson HR, Ferguson J et al. Reciprocal priming between receptor tyrosine kinases at recycling endosomes orchestrates cellular signalling outputs The EMBO Journal 2021-07-15 [PMID: 34086370]

Bioinformatics

• Gene Symbol: RAB11FIP1
• Uniprot:
  • Q6WKZ4(Human)