Rab25 Antibody (3F12)

Images

 
Western Blot: Rab25 Antibody (3F12) [NBP1-51599] - Western blot analysis using Rab25 mouse mAb against MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: Rab25 Antibody (3F12) [NBP1-51599] - Immunofluorescence analysis of A549 cells using RAB25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
Immunohistochemistry-Paraffin: Rab25 Antibody (3F12) [NBP1-51599] - Immunohistochemical analysis of paraffin-embedded esophagus tissues (left) and human lung cancer (right) using Rab25 mouse mAb with DAB staining.
Flow Cytometry: Rab25 Antibody (3F12) [NBP1-51599] - Flow cytometric analysis of NIH/3T3 cells using Rab25 mouse mAb (green) and negative control (purple).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
3F12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Rab25 Antibody (3F12) Summary

Immunogen
Purified recombinant fragment of Rab25 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RAB25
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Rab25 Antibody (3F12)

  • CATX8
  • CATX-8RAB11C
  • RAB11C
  • Rab25
  • RAB25, member RAS oncogene family
  • ras-related protein Rab-25

Background

Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-49320
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
H00339122-M01
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP2-52546
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, ICC, IF
NBP2-15085
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB500-418
Species: Hu, Po, Rb
Applications: WB, B/N, ELISA, ICC/IF, IHC, IHC-P, RIA
NBP1-91991
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-92307
Species: Hu, Rt
Applications: WB, IHC, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
H00338382-M01
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC-P, IP
H00005956-R02
Species: Hu
NBP1-87746
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-05048
Species: Hu, Mu, Rt, Bv, Ca, Sh
Applications: WB, ICC/IF
NB300-595
Species: Mu, Rt, Bv
Applications: WB, Flow, IHC, IHC-P, IP, IF
NBP1-81855
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB120-13253
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-82949
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-84495
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-41187
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
H00004218-M02
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP1-51599
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P

Publications for Rab25 Antibody (NBP1-51599) (0)

There are no publications for Rab25 Antibody (NBP1-51599).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Rab25 Antibody (NBP1-51599) (0)

There are no reviews for Rab25 Antibody (NBP1-51599). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rab25 Antibody (NBP1-51599) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Rab25 Products

Bioinformatics Tool for Rab25 Antibody (NBP1-51599)

Discover related pathways, diseases and genes to Rab25 Antibody (NBP1-51599). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Rab25 Antibody (NBP1-51599)

Discover more about diseases related to Rab25 Antibody (NBP1-51599).
 

Pathways for Rab25 Antibody (NBP1-51599)

View related products by pathway.

PTMs for Rab25 Antibody (NBP1-51599)

Learn more about PTMs related to Rab25 Antibody (NBP1-51599).

Blogs on Rab25

There are no specific blogs for Rab25, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Rab25 Antibody (3F12) and receive a gift card or discount.

Bioinformatics

Gene Symbol RAB25
Entrez