Rab25 Antibody (3F12) - BSA Free

Images

 
Western Blot: Rab25 Antibody (3F12) [NBP1-51599] - Analysis using Rab25 mouse mAb against MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: Rab25 Antibody (3F12) [NBP1-51599] - Analysis of A549 cells using RAB25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Immunohistochemistry-Paraffin: Rab25 Antibody (3F12) [NBP1-51599] - Analysis of esophagus tissues (left) and human lung cancer (right) using Rab25 mouse mAb with DAB staining.
Flow Cytometry: Rab25 Antibody (3F12) [NBP1-51599] - Analysis of NIH/3T3 cells using Rab25 mouse mAb (green) and negative control (purple).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, KD
Clone
3F12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

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Catalog# & Formulation Size Price

Rab25 Antibody (3F12) - BSA Free Summary

Immunogen
Purified recombinant fragment of Rab25 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RAB25
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Knockdown Validated
  • Western Blot 1:500-1:2000
Application Notes
Knockdown validation (PMID: 31564077).
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-51599 in the following applications:

  • 1 publication
  • KD
    1 publication
  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Rab25 Antibody (3F12) - BSA Free

  • CATX8
  • CATX-8RAB11C
  • RAB11C
  • Rab25
  • RAB25, member RAS oncogene family
  • ras-related protein Rab-25

Background

Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rab25 Antibody (NBP1-51599)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: IF/IHC, KD, WB.


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IF/IHC
(1)
KD
(1)
WB
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RAB25