Urocortin 3 Antibody

Western Blot: Urocortin 3 Antibody [NB100-93444] - HEK293 lysate (10ug protein in RIPA buffer) over expressing Human UCN3 with DYKDDDDK tag probed with NB100-93444 (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with NB100-93444 (1mg/ml) in Lane B.

• Reactivity: Hu
• Applications: WB, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Urocortin 3 Antibody Summary

• Immunogen: Peptide with sequence C-KPRQDTAKSPHRTK corresponding to internal region according to NP_444277.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UCN3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 - 2 ug/ml
  • Peptide ELISA Detection limit 1:16000
• Application Notes: WB: In transfected HEK293 transiently expressing full-length human UCN3 (myc and DYKDDDDK tagged), a band of approx. 19 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Urocortin 3 Antibody

• MGC119002
• SPCSCP
• stresscopin
• Ucn III
• UCNIII
• urocortin 3 (stresscopin)
• Urocortin III
• urocortin-3

Background

This gene is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Urocortin 3 Antibody (NB100-93444)(1)

Publication using NB100-93444

• Jiang Z, Michal JJ, Williams GA et al. Cross species association examination of UCN3 and CRHR2 as potential pharmacological targets for antiobesity drugs. PLoS ONE 2006 Dec 20 [PMID: 17183713]

Bioinformatics

• Gene Symbol: UCN3
• Entrez:
  • 114131(Human)
• Uniprot:
  • Q969E3(Human)