Filamin A Antibody (SA30-08)

Western Blot: Filamin A Antibody (SA30-08) [NBP2-67304] - Analysis of Filamin A on different cell lysates using anti-Filamin A antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Jurkat Lane 3: Hela Lane 4: NIH/3T3

Immunocytochemistry/Immunofluorescence: Filamin A Antibody (SA30-08) [NBP2-67304] - Staining Filamin A in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Filamin A Antibody (SA30-08) [NBP2-67304] - Analysis of paraffin-embedded mouse uterus tissue using anti-Filamin A antibody. Counter stained with hematoxylin.

Flow Cytometry: Filamin A Antibody (SA30-08) [NBP2-67304] - Analysis of Hela cells with Filamin A antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Filamin A Antibody (SA30-08) [NBP2-67304] - Staining Filamin A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Filamin A Antibody (SA30-08) [NBP2-67304] - Staining Filamin A in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Filamin A Antibody (SA30-08) [NBP2-67304] - Analysis of paraffin-embedded human uterus tissue using anti-Filamin A antibody. Counter stained with hematoxylin.

Product Discontinued

Filamin A Antibody (SA30-08) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Filamin A aa 2,598-2,647 / 2,647. (SwissProt: P21333 Human; SwissProt: Q8BTM8 Mouse; Gene ID: 293860 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FLNA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Filamin A Antibody (SA30-08)

• ABP-280
• ABP-280DKFZp434P031
• ABPX
• actin binding protein 280
• Actin-binding protein 280
• Alpha-filamin
• CVD1
• EC 2.1.1.43
• EC 6.3.4.4
• Endothelial actin-binding protein
• Filamin A
• filamin A, alpha (actin binding protein 280)
• filamin A, alpha
• Filamin-1
• filamin-A
• FLN
• FLN1
• FLN1OPD
• FLNA
• FLN-A
• FMD
• MNS
• NHBP
• Non-muscle filamin
• OPD1
• OPD2
• XLVD
• XMVD

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FLNA