Ryanodine Receptor Antibody (34C) - BSA Free

Immunohistochemistry-Paraffin: Ryanodine Receptor Antibody (34C) [NB300-543] - Biopsies of deparaffinized Human skeletal muscle tissue.

• Reactivity: Hu, Mu, Rt, Am, Bv, Ca, Fi, Ma, Ma-Mn, Pm, Rb, Sh
• Applications: WB, ICC/IF, IHC, IP, B/N
• Clone: 34C
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Ryanodine Receptor Antibody (34C) - BSA Free Summary

• Description: Novus Biologicals Mouse Ryanodine Receptor Antibody (34C) - BSA Free (NB300-543) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-Ryanodine Receptor Antibody: Cited in 11 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Partially purified chicken pectoral muscle ryanodine receptor.
• Specificity: This antibody detects ryanodine receptor Ryanodine Receptor-1 and Ryanodine Receptor-2 isoforms in amphibian, chicken, bovine, canine, fish, human, non-human primate, mouse, rabbit, mink, rat and sheep tissues. In chicken, it detects the alpha, beta and cardiac isoforms. This antibody also detects Ryanodine Receptor-3 in mouse cells. In frog, it detects the alpha and beta isoforms and in fish it detects the alpha isoform.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RYR1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:5000
• Application Notes: May be useful in IF, IHC-Fr, IP, and WB. WB: Detects an approx. 565 kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. IHC: Staining of RyR in chicken brain results in intense staining of cerebral Purkinje neurons. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 15564379).
• Theoretical MW: 565 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Ryanodine Receptor Antibody (34C) - BSA Free

• ARVC2
• ARVD2
• cardiac muscle ryanodine receptor-calcium release channel
• cardiac-type ryanodine receptor
• CCO
• central core disease of muscle
• islet-type ryanodine receptor
• kidney-type ryanodine receptor
• MHS
• MHS1
• PPP1R137
• protein phosphatase 1, regulatory subunit 137
• ryanodine receptor 1 (skeletal)
• ryanodine receptor 1
• ryanodine receptor 2 (cardiac)
• ryanodine receptor 2
• RYDR
• RYR
• RYR-1
• RYR-2
• sarcoplasmic reticulum calcium release channel
• Skeletal muscle calcium release channel
• skeletal muscle ryanodine receptor
• SKRR
• type 1-like ryanodine receptor
• type 2 ryanodine receptor
• VTSIP

Background

The Ryanodine Receptor (RyR) is the channel responsible for calcium release from muscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR. Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ryanodine Receptor Antibody (NB300-543)(12)

Publications using NB300-543

• Jordan T et al. Inhibition of ryanodine receptor 1 in fast skeletal muscle fibers induces a fast-to-slow muscle fiber type transition. J Cell Sci; 117(Pt 25):6175-83. 2004-12-01 [PMID: 15564379] (ICC/IF)
• Liu Z et al. Three-dimensional reconstruction of the recombinant type 3 ryanodine receptor localization of its amino terminus. Proc Natl Acad Sci U S A 98:6104-9. 2001-01-01 [PMID: 11353864]
• Yamada KA et al. Long-chain acylcarnitine induces Ca2+ efflux from the sarcoplasmic reticulum. J Cardiovasc Pharmacol 36:14-21. 2000-01-01 [PMID: 10892655]
• Chan SL et al. Presenilin-1 mutations increase levels of ryanodine receptors calcium release in PC12 cells cortical neurons. J Biol Chem 275:18195-200. 2000-01-01 [PMID: 10764737]
• Moore RA et al. A transgenic myogenic cell line lacking ryanodine receptor protein for homologous expression studies: reconstitution of Ry1R protein function. J Cell Biol 140:843-51. 1998-01-01 [PMID: 9472036]
• Protasi F et al. Role of ryanodine receptors in the assembly of calcium release units in skeletal muscle. J Cell Biol 140:831-42. 1998-01-01 [PMID: 9472035]
• Chen SR et al. Positioning of major tryptic fragments in the Ca2+ release channel (ryanodine receptor) resulting from partial digestion of rabbit skeletal muscle sarcoplasmic reticulum. J Biol Chem 268:22642-9. 1993-01-01 [PMID: 8226772]
• Liu G et al. Molecular interaction between ryanodine receptor and glycoprotein triadin involves redox cycling of functionally important hyperreactive sulfhydryls. J Biol Chem 269 (52):33028-34. 1994-12-30 [PMID: 7806531] (B/N)
• Naukkarinen A. Myotonic dystrophy type 2 (DM2): Diagnostic methods and molecular pathology . 2011; University of Helsinki, Finland.
• Airey JA et al. Three ryanodine receptor isoforms exist in avian striated muscles. Biochemistry 32:5739-45. 1993-01-01 [PMID: 7684927]
• Airey JA et al. Identification localization of two triad junctional foot protein isoforms in mature avian fast twitch skeletal muscle. J Biol Chem 265:14187-94. 1990-01-01 [PMID: 2387846]
• Walton PD et al. Ryanodine inositol trisphosphate receptors coexist in avian cerebellar Purkinje neurons. J Cell Biol 113:1145-57. 1991-01-01 [PMID: 1645737]

Bioinformatics

• Gene Symbol: RYR1
• Entrez:
  • 20190(Mouse)
  • 114207(Rat)
• Uniprot:
  • Q90984(Chicken)