Ryanodine Receptor Antibody (34C)

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Immunohistochemistry-Paraffin: Ryanodine Receptor Antibody (34C) [NB300-543] - Biopsies of deparaffinized Human skeletal muscle tissue.

Product Details

Summary
Reactivity Hu, Mu, Rt, Am, Bv, Ca, Fi, Ma, Ma-Mn, Pm, Rb, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N
Clone
34C
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Ryanodine Receptor Antibody (34C) Summary

Immunogen
Partially purified chicken pectoral muscle ryanodine receptor.
Specificity
This antibody detects ryanodine receptor Ryanodine Receptor-1 and Ryanodine Receptor-2 isoforms in amphibian, chicken, bovine, canine, fish, human, non-human primate, mouse, rabbit, mink, rat and sheep tissues. In chicken, it detects the alpha, beta and cardiac isoforms. This antibody also detects Ryanodine Receptor-3 in mouse cells. In frog, it detects the alpha and beta isoforms and in fish it detects the alpha isoform.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RYR1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Block/Neutralize
Application Notes
May be useful in IF, IHC-Fr, IP, and WB. WB: Detects an approx. 565 kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. IHC: Staining of RyR in chicken brain results in intense staining of cerebral Purkinje neurons. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 15564379).
Theoretical MW
565 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB300-543.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Ryanodine Receptor Antibody (34C)

  • ARVC2
  • ARVD2
  • cardiac muscle ryanodine receptor-calcium release channel
  • cardiac-type ryanodine receptor
  • CCO
  • central core disease of muscle
  • islet-type ryanodine receptor
  • kidney-type ryanodine receptor
  • MHS
  • MHS1
  • PPP1R137
  • protein phosphatase 1, regulatory subunit 137
  • ryanodine receptor 1 (skeletal)
  • ryanodine receptor 1
  • ryanodine receptor 2 (cardiac)
  • ryanodine receptor 2
  • RYDR
  • RYR
  • RYR-1
  • RYR-2
  • sarcoplasmic reticulum calcium release channel
  • Skeletal muscle calcium release channel
  • skeletal muscle ryanodine receptor
  • SKRR
  • type 1-like ryanodine receptor
  • type 2 ryanodine receptor
  • VTSIP

Background

The Ryanodine Receptor (RyR) is the channel responsible for calcium release from muscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR. Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ryanodine Receptor Antibody (NB300-543)(12)

We have publications tested in 2 applications: B/N, ICC/IF.


Filter By Application
B/N
(1)
ICC/IF
(1)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB300-543 Applications Species
Jordan T et al. Inhibition of ryanodine receptor 1 in fast skeletal muscle fibers induces a fast-to-slow muscle fiber type transition. J Cell Sci; 117(Pt 25):6175-83. 2004 Dec 1. [PMID: 15564379] (ICC/IF) ICC/IF
Liu Z et al. Three-dimensional reconstruction of the recombinant type 3 ryanodine receptor localization of its amino terminus. Proc Natl Acad Sci U S A 98:6104-9. 2001 [PMID: 11353864]
Yamada KA et al. Long-chain acylcarnitine induces Ca2+ efflux from the sarcoplasmic reticulum. J Cardiovasc Pharmacol 36:14-21. 2000 [PMID: 10892655]
Chan SL et al. Presenilin-1 mutations increase levels of ryanodine receptors calcium release in PC12 cells cortical neurons. J Biol Chem 275:18195-200. 2000 [PMID: 10764737]
Moore RA et al. A transgenic myogenic cell line lacking ryanodine receptor protein for homologous expression studies: reconstitution of Ry1R protein function. J Cell Biol 140:843-51. 1998 [PMID: 9472036]
Protasi F et al. Role of ryanodine receptors in the assembly of calcium release units in skeletal muscle. J Cell Biol 140:831-42. 1998 [PMID: 9472035]
Chen SR et al. Positioning of major tryptic fragments in the Ca2+ release channel (ryanodine receptor) resulting from partial digestion of rabbit skeletal muscle sarcoplasmic reticulum. J Biol Chem 268:22642-9. 1993 [PMID: 8226772]
Liu G et al. Molecular interaction between ryanodine receptor and glycoprotein triadin involves redox cycling of functionally important hyperreactive sulfhydryls. J Biol Chem 269 (52):33028-34. 1994 Dec 30. [PMID: 7806531] (B/N) B/N
Airey JA et al. Three ryanodine receptor isoforms exist in avian striated muscles. Biochemistry 32:5739-45. 1993 [PMID: 7684927]
Airey JA et al. Identification localization of two triad junctional foot protein isoforms in mature avian fast twitch skeletal muscle. J Biol Chem 265:14187-94. 1990 [PMID: 2387846]
Show All 12 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ryanodine Receptor Antibody (NB300-543) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB300-543AF350
Alexa Fluor 405 NB300-543AF405
Alexa Fluor 488 NB300-543AF488
Alexa Fluor 532 NB300-543AF532
Alexa Fluor 594 NB300-543AF594
Alexa Fluor 647 NB300-543AF647
Alexa Fluor 700 NB300-543AF700
Alexa Fluor 750 NB300-543AF750
Biotin NB300-543B
DyLight 350 NB300-543UV
DyLight 405 NB300-543V
DyLight 488 NB300-543G
DyLight 550 NB300-543R
DyLight 594 NB300-543DL594
DyLight 650 NB300-543C
DyLight 680 NB300-543FR
DyLight 755 NB300-543IR
FITC NB300-543F
HRP NB300-543H
Janelia Fluor 549 NB300-543JF549
Janelia Fluor 646 NB300-543JF646

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Bioinformatics

Gene Symbol RYR1
Entrez
Uniprot