FIG4 Antibody (SR2515)

Western Blot: FIG4 Antibody (SR2515) [NBP3-27791] - Western blot analysis of FIG4 in HL-60, U2OS, U87-MG lysates

• Reactivity: Hu
• Applications: WB, IP
• Clone: SR2515
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

FIG4 Antibody (SR2515) Summary

• Description: Novus Biologicals Rabbit FIG4 Antibody (SR2515) (NBP3-27791) is a recombinant monoclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human FIG4 (Uniprot #: Q92562)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FIG4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:20
  • Western Blot 1:500-1:1000
• Theoretical MW: 104 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for FIG4 Antibody (SR2515)

• ALS11polyphosphoinositide phosphatase
• CMT4J
• dJ249I4.1
• EC 3.1.3
• EC 3.1.3.-
• EC 3.1.3.36
• FIG4 homolog (S. cerevisiae)
• FIG4 homolog, SAC1 lipid phosphatase domain containing (S. cerevisiae)
• hSac3
• KIAA0274FIG4 homolog, SAC domain containing lipid phosphatase
• Phosphatidylinositol 3,5-bisphosphate 5-phosphatase
• Sac domain-containing inositol phosphatase 3
• SAC domain-containing protein 3
• SAC3FIG4 homolog, SAC1 domain containing lipid phosphatase

Background

The protein encoded by the FIG4 gene belongs to the SAC domain-containing protein gene family. The SAC domain, approximately 400 amino acids in length and consisting of seven conserved motifs, has been shown to possess phosphoinositide phosphatase activity. The yeast homolog, Sac1p, is involved in the regulation of various phosphoinositides, and affects diverse cellular functions such as actin cytoskeleton organization, Golgi function, and maintenance of vacuole morphology. Membrane-bound phosphoinositides function as signaling molecules and play a key role in vesicle trafficking in eukaryotic cells. Mutations in this gene have been associated with Charcot-Marie-Tooth disease, type 4J. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FIG4