MCM3AP Antibody - BSA Free

Western Blot: MCM3AP Antibody - BSA Free [NBP3-46263] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (MCM3AP antibody) at dilution of 1:300

Immunoprecipitation: MCM3AP Antibody - BSA Free [NBP3-46263] - IP Result of anti-MCM3AP (IP:, 4ug; Detection: 1:500) with mouse brain tissue lysate 4000ug.

Immunohistochemistry: MCM3AP Antibody - BSA Free [NBP3-46263] - Immunohistochemistry of paraffin-embedded human colon cancer using (MCM3AP antibody) at dilution of 1:50

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MCM3AP Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MCM3AP Antibody - BSA Free (NBP3-46263) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: minichromosome maintenance complex component 3 associated protein. (Uniprot# O60318)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MCM3AP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Immunohistochemistry 1:10-1:100
  • Immunoprecipitation 1:500-1:1000
  • Western Blot 1:200-1:1000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MCM3AP Antibody - BSA Free

• 80 kDa MCM3-associated protein
• FLJ44336
• GANPFLJ45306
• human mRNA for MCM3 import factor10Protein GANP
• KIAA0572MAP80germinal center-associated nuclear protein
• Map80
• MCM3 import protein
• MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) associated protein
• MCM3 minichromosome maintenance deficient 3 associated protein
• minichromosome maintenance complex component 3 associated protein
• minichromosome maintenance deficient (S. cerevisiae) 3-associated protein
• minichromosome maintenance deficient 3-associated protein

Background

The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation of the MCM complex (composed of at least MCM proteins 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MCM3AP