ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (511702)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 511702
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (511702) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ASAHL/N‑acylethanolamine-hydrolyzing Acid A isoform 1
  Ser29-Lys359 (Val151Ile)
  Accession # Q02083
• Specificity: Detects human ASAHL/N‑acylethanolamine-hydrolyzing Acid A in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse ASAHL is observed and no cross-reactivity with recombinant human ASAH2 or recombinant mouse ASAH2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NAAA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (511702)

• Acid ceramidase-like protein
• ASAHL
• ASAH-like protein
• EC 3.5.1.-
• NAAA
• N-acylethanolamine acid amidase
• Nacylethanolaminehydrolyzing Acid Amidase
• N-acylethanolamine-hydrolyzing acid amidase
• N-acylsphingosine amidohydrolase (acid ceramidase)-like
• N-acylsphingosine amidohydrolase-like
• PLT

Background

The human NAAA gene encodes N-acylethanolamine-hydrolyzing acid amidase, also known as ASAH-like protein (ASAHL), a fatty acid amidase with maximal activity at acidic pH (1). ASAHL hydrolyzes a number of N-acyl ethanolamines, including N-myristoyl-, N-stearoyl, N-oleoyl, and N-arachidonoyl, but is most active against N‑palmitoylethanolamine (2). ASAHL is a member of the cholylglycine hydrolase family of enzymes, and is structurally similar to acid ceramidase (3). ASAHL is both a lysosomal and a secreted enzyme, and like acid ceramidase, has been observed to be proteolytically processed during maturation (3). ASAHL can be distinguished from anandamide amidohydrolase by its lack of inhibition by methyl arachidonyl fluorophosphonate (2).

1. Hong, S.B. et al. (1999) Genomics 62:232.
2. Ueda, N. et al. (2001) J. Biol. Chem. 276:35552.
3. Tsuboi, K. et al. (2005) J. Biol. Chem. 280:11082.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (MAB4494)(3)

Publications using MAB4494

• Mulè, S;Galla, R;Ferrari, S;Invernizzi, M;Uberti, F; Molecular Insights into the Nociceptive Modulation by Palmitoylethanolamide and Equisetum arvense Extract: An In Vitro Study Across the Blood-Brain Barrier Nutrients 2025-06-13 [PMID: 40573109] (Western Blot, Human)
• Oscar Sasso, Guillermo Moreno-Sanz, Cataldo Martucci, Natalia Realini, Mauro Dionisi, Luisa Mengatto et al. Antinociceptive effects of the N-acylethanolamine acid amidase inhibitor ARN077 in rodent pain models Pain 2013-03-01 [PMID: 23218523]
• Oscar Sasso, Guillermo Moreno-Sanz, Cataldo Martucci, Natalia Realini, Mauro Dionisi, Luisa Mengatto et al. Antinociceptive effects of the N-acylethanolamine acid amidase inhibitor ARN077 in rodent pain models Pain 2013-03-01 [PMID: 23218523] (Western Blot, Human)

Bioinformatics

• Gene Symbol: NAAA
• Uniprot:
  • Q02083()