Ficolin-3 Isoform 2 Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Ficolin-3 Isoform 2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Ficolin-3
  Lys22-Arg299
  Accession # O75636-1
• Specificity: Detects human Ficolin-3 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Ficolin-1 is observed and less 1% cross-reactivity with rhFicolin-2 and rhTenascinR is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FCN3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ficolin-3 Isoform 2 Antibody [Unconjugated]

• FCN3
• FCNH
• Ficolin3
• Ficolin-3
• HAKA1
• H-Ficolin

Background

Human Ficolin-3 (fibrinogen/collagen-like), also called H-ficolin and, previously, Hakata antigen or thermolabile beta -2 macroglycoprotein, is a member of the ficolin family of secreted pattern recognition proteins that belong to the lectin complement activation pathway (1, 2). Ficolin-3 is expressed by bile duct epithelial cells and hepatocytes, and is released into the bile and circulation, where it averages 18 μg/mL (3, 4). It is also secreted by bronchial and alveolar epithelial cells in the lung (3). Mature human Ficolin-3 shares 46% and 52% amino acid (aa) identity with human Ficolin-1 and Ficolin-2, respectively. Ficolin-3 has only been identified in primates and is likely a pseudogene in other species (5). The 35 kDa, 288 aa human Ficolin-3 (isoform 2) contains a signal sequence, an N-terminal collagen domain and a C-terminal fibrinogen-like domain that includes a calcium binding site and two potential N-glycosylation sites. Isoform 1 contains an additional 11 aa between the collagen and fibrinogen-like domains. The collagen domain mediates trimer formation, and a ~650 kDa, 18 subunit oligomer is formed by disulfide links at the N-terminus (2, 6, 7). Ficolin-3 binds a limited set of carbohydrates containing mannose, galactose or D-fucose (2, 6). Binding of microbial carbohydrates has been clearly demonstrated only for the PSA antigen of Aerococcus viridans (4, 8, 9). Pathogen recognition initiates an immune response involving the calcium-dependent interaction of Ficolin-3 with the MBL-associated serine protease (MASP) complex. This cleaves C4 to activate the complement pathway (4, 9). In a secondary role, Ficolins 2 and 3 bind apoptotic cells, activating complement cascades that assist in clearance of the cells (10). Circulating antibodies to Ficolin-3 have been identified in systemic lupus erythematosus (7).

1. Endo, Y. et al. (2006) Adv. Exp. Med. Biol. 586:265.
2. Sugimoto, R. et al. (1998) J. Biol. Chem. 273:20721.
3. Akaiwa, M. et al. (1999) J. Histochem. Cytochem. 47:777.
4. Krarup, A. et al. (2005) Inf. Immun. 72:1052.
5. Endo, Y. et al. (2004) Genomics 84:737.
6. Garlatti, V. et al. (2007) EMBO J. 26:623.
7. Yae, Y. et al. (1991) Biochim. Biophys. Acta 1078:369.
8. Tsujimura, M. et al. (2001) Clin. Diag. Lab. Immunol. 8:454.
9. Matsushita, M. et al. (2002) J. Immunol. 168:3502.
10. Kuraya, M. et al. (2005) Immunobiology 209:689.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ficolin-3 Isoform 2 Antibody (AF2367)(2)

Publications using AF2367

• H Jang, Y Jun, S Kim, E Kim, Y Jung, BJ Park, J Lee, J Kim, S Lee, J Kim FCN3 functions as a tumor suppressor of lung adenocarcinoma through induction of endoplasmic reticulum stress Cell Death & Disease, 2021-04-15;12(4):407. 2021-04-15 [PMID: 33859174] (Western Blot, Human)
• Søren E. Degn, Lisbeth Jensen, Tomasz Olszowski, Jens C. Jensenius, Steffen Thiel Co-Complexes of MASP-1 and MASP-2 Associated with the Soluble Pattern-Recognition Molecules Drive Lectin Pathway Activation in a Manner Inhibitable by MAp44 The Journal of Immunology 2013-08-01 [PMID: 23785123]

Bioinformatics

• Gene Symbol: FCN3
• Uniprot:
  • O75636-1()