Ficolin-1 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

Ficolin-1 Antibody [Alexa Fluor® 405] Summary

• Specificity: Detects human Ficolin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human (rh) Ficolin-2 is observed and less than 1% cross-reactivity with rhFicolin-
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ficolin-1 Antibody [Alexa Fluor® 405]

• Collagen/fibrinogen domain-containing protein 1
• FCN1
• Fcna
• FCNM
• FCNMficolin-A
• ficolin (collagen/fibrinogen domain containing) 1
• ficolin (collagen/fibrinogen domain-containing) 1
• Ficolin1
• Ficolin-1
• Ficolin-A
• ficolin-alpha
• M-ficolin

Background

Human Ficolin-1 (fibrinogen/collagen-like), also called M-ficolin, is a member of the ficolin family of secreted pattern recognition proteins in the lectin complement activation pathway (1‑3). Ficolin-1 is expressed by monocytes, neutrophils and type II alveolar epithelial cells (2‑5). It is proposed to be a locally-acting lectin released in a regulated manner (4). Ficolin-1 is not found in plasma, but is detected on the surface of circulating monocytes (4‑6). The 35 kDa, 326 amino acid (aa) human Ficolin-1 contains a 28 aa signal sequence, an N-terminal collagen domain and a C-terminal fibrinogen-like (FBG) domain that includes a calcium binding site and one potential N-glycosylation site. Both the collagen and FBG domains mediate trimer formation (7). Like Ficolin-2, larger homo-multimers of Ficolin-1 exist and are likely formed by disulfide bonds at the N-terminus. Sizes corresponding to 12 or 18 subunit oligomers are reported (4, 6, 7). The FBG domain of Ficolin-1 binds microbial ligands that contain acetylated compounds (6). Ligands identified include N-acetyl glucosamine, N-acetyl galactosamine and sialyl-N-acetyllactosamine (4‑7). Like other ficolins, Ficolin-1 associates with, and activates the MBL-associated serine protease (MASP) complex, which activates the complement pathway by cleaving C4, contributing to the innate immune response (4‑6). Mature human Ficolin-1 shares 76%, 61%, 61%, 76% and 81% aa identity with mouse Ficolin-2 (8), and mouse, rat, canine and porcine Ficolin-1, respectively. It shares 84% and 46% aa identity with human Ficolin-2 and Ficolin-3, respectively. The Ficolin-1 gene is polymorphic, showing at least ten single nucleotide polymorphisms in the promoter and coding regions (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.