Fibulin 5/DANCE Antibody (2424)

Western Blot: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - Analysis of Fibulin 5 on human skin tissue lysate using anti-Fibulin 5 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - ICC staining Fibulin 5 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - Mouse ovary tissue using anti-Fibulin 5 antibody. Counter stained with hematoxylin.

Flow Cytometry: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - Flow cytometric analysis of SiHa cells with Fibulin 5 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - ICC staining Fibulin 5 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - ICC staining Fibulin 5 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - Rat testis tissue using anti-Fibulin 5 antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: 2424
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Fibulin 5/DANCE Antibody (2424) Summary

• Description: Novus Biologicals Rabbit Fibulin 5/DANCE Antibody (2424) (NBP2-76929) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein within Human Fibulin 5/DANCE aa 203-378 / 448. (SwissProt: Q9UBX5 Human; SwissProt: Q9WVH9 Mouse; SwissProt: Q9WVH8 Rat)
• Localization: Extracellular matrix. Secreted.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FBLN5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Fibulin 5/DANCE Antibody (2424)

• ARMD3fibulin-5
• DANCE
• DANCEFIBL-5
• Developmental arteries and neural crest EGF-like protein
• EVEC
• FBLN5
• Fibulin 5
• UP50
• UP50FLJ90059
• Urine p50 protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FBLN5