Fibulin-3/EFEMP1 Antibody

Western Blot: EFEMP1 Antibody [NB300-691] - 62 kDa EFEMP1from human RPE cells. Antibody diluted 1:500

Immunohistochemistry-Paraffin: Fibulin-3/EFEMP1 Antibody [NB300-691] - 1:50 dilution, DAB (brown) staining and Hematoxyin QS (blue) counterstain. 40X magnification, FFPE section

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Fibulin-3/EFEMP1 Antibody Summary

• Description: Novus Biologicals Rabbit Fibulin-3/EFEMP1 Antibody (NB300-691) is a polyclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic cyclic peptide corresponding to N terminal amino acids 25-42 of Human EFEMP1, peptide was covalently modified. Sequence: TYTQCTDGYEWDPVRQQC
• Localization: Secreted
• Specificity: The Anti EFEMP1 selective was generated against a conserved sequence near the N terminal region that is unique to EFEMP1 protein only. This detects a strong 62 kDa EFEMP1 protein in human.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EFEMP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:200
  • Western Blot 1:500
• Application Notes: Detects a band of approximately 62 kDa (predicted molecular weight: 60 kDa). Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Fibulin-3/EFEMP1 Antibody

• DHRD
• EFEMP1
• EGF containing fibulin-like extracellular matrix protein 1
• EGF-containing fibulin-like extracellular matrix protein 1
• Extracellular protein S1-5
• FBLN3
• FBLN3DRAD
• FBNLFLJ35535
• FIBL-3
• Fibrillin-like protein
• fibrillin-like
• Fibulin 3
• fibulin-3
• MGC111353
• MLVT
• MTLV
• S1-5

Background

This gene spans approximately 18 kb of genomic DNA and consists of 12 exons. Two transcripts with distinct 5' UTR have been described; the resulting proteins have distinct N-terminal amino acid sequences. Translation initiation from internal methionine residues was observed with in vitro translation. A signal peptide sequence is predicted for translation initiation sites 1, 2, and 4. The protein isoforms contain 5 or 6 calcium-binding EGF2 domains and 5 or 6 EGF2 domains. Mutations in this gene cause the retinal disease Malattia Leventinese.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Fibulin-3/EFEMP1 Antibody (NB300-691). (Showing 1 - 1 of 1 FAQ).

1. May I know the exact formulation for antibody NB300-691 (EFEMP1 antibody)? Other than the 0.02% sodium azide, are there other components like buffer salts, BSA, etc? I am primarily concerned with the possible presence of BSA, Tris, glycine, or gelatin.
   • NB300-691 is supplied in Tris/glycine buffer at pH 7.4 containing 0.02% sodium azide as preservative, cryoprotective agents, stabilizing proteins (BSA) and glycerol.

Bioinformatics

• Gene Symbol: EFEMP1
• Entrez:
  • 2202(Human)
  • 305604(Rat)