Fibulin-3/EFEMP1 Antibody

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Western Blot: EFEMP1 Antibody [NB300-691] - 62 kDa EFEMP1from human RPE cells. Antibody diluted 1:500

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Fibulin-3/EFEMP1 Antibody Summary

Immunogen
Synthetic peptide (N-term): TYTQCTDGYEWDPVRQQC.
Localization
Secreted
Specificity
The Anti EFEMP1 selective was generated against a conserved sequence near the N terminal region that is unique to EFEMP1 protein only. This detects a strong 62 kDa EFEMP1 protein in human.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EFEMP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
IP: Use at a dilution of 1/200. WB: Use at a dilution of 1/500. Detects a band of approximately 62 kDa (predicted molecular weight: 60 kDa). Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Positive Control
Fibulin-3/EFEMP1 Lysate (NBL1-10135)

Reactivity Notes

Cross-reacts with Human and Rat. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Fibulin-3/EFEMP1 Antibody

  • DHRD
  • EFEMP1
  • EGF containing fibulin-like extracellular matrix protein 1
  • EGF-containing fibulin-like extracellular matrix protein 1
  • Extracellular protein S1-5
  • FBLN3
  • FBLN3DRAD
  • FBNLFLJ35535
  • FIBL-3
  • Fibrillin-like protein
  • fibrillin-like
  • Fibulin 3
  • fibulin-3
  • MGC111353
  • MLVT
  • MTLV
  • S1-5

Background

This gene spans approximately 18 kb of genomic DNA and consists of 12 exons. Two transcripts with distinct 5' UTR have been described; the resulting proteins have distinct N-terminal amino acid sequences. Translation initiation from internal methionine residues was observed with in vitro translation. A signal peptide sequence is predicted for translation initiation sites 1, 2, and 4. The protein isoforms contain 5 or 6 calcium-binding EGF2 domains and 5 or 6 EGF2 domains. Mutations in this gene cause the retinal disease Malattia Leventinese.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Fibulin-3/EFEMP1 Antibody (NB300-691). (Showing 1 - 1 of 1 FAQ).

  1. May I know the exact formulation for antibody NB300-691 (EFEMP1 antibody)? Other than the 0.02% sodium azide, are there other components like buffer salts, BSA, etc? I am primarily concerned with the possible presence of BSA, Tris, glycine, or gelatin.
    • NB300-691 is supplied in Tris/glycine buffer at pH 7.4 containing 0.02% sodium azide as preservative, cryoprotective agents, stabilizing proteins (BSA) and glycerol.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EFEMP1
Entrez