Fibulin 1 Antibody (CL0337) - Azide and BSA Free

Analysis in human cervix, uterine and liver tissues using NBP3-43893 antibody. Corresponding Fibulin 1 RNA-seq data are presented for the same tissues.

Lane 1: Marker [kDa]Lane 2: Human plasma

Staining of human breast cancer shows moderate positivity in the extracellular matrix of tumor stroma.

Staining of human liver shows no positivity as expected.

Staining of human prostate shows moderate positivity in the extracellular matrix.

Staining of MCF7 cells using the Anti-Fibulin 1 monoclonal antibody) .

Staining of RT-4 cells using the Anti-Fibulin 1 monoclonal antibody) .

Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-Fibulin 1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Staining of human uterine cervix shows strong positivity in the extracellular matrix.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL0337
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Fibulin 1 Antibody (CL0337) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Fibulin 1 Antibody (CL0337) - Azide and BSA Free (NBP2-52918) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P23142, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (92%), Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FBLN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Fibulin 1 Antibody (CL0337) - Azide and BSA Free

• FBLN
• FBLN1
• FIBL1
• FIBL-1
• Fibulin 1
• fibulin-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FBLN1