Testican 1/SPOCK1 Antibody (316801) [Unconjugated]

• Applications: WB, IP
• Clone: 316801
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Testican 1/SPOCK1 Antibody (316801) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Testican 1/SPOCK1
  Arg22-Trp439
  Accession # Q08629
• Specificity: Detects human Testican 1/SPOCK1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Testican 2 or rhTestican 3 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPOCK1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Testican 1/SPOCK1 Antibody (316801) [Unconjugated]

• FLJ37170
• Protein SPOCK
• sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1
• SPOCK1
• SPOCKcwcv and kazal-like domains proteoglycan (testican)
• Testican 1
• TIC1
• TICN1

Background

Testican 1, also known as SPOCK1, was originally identified in seminal plasma and is also expressed in brain. It is a proteoglycan that contains a Ca2+-binding domain and a C-terminal acidic domain with putative glycosaminoglycan attachment sites as well as three potential inhibitory domains targeted toward three different classes of proteases, metallo-, cysteine and serine proteases. Human Testican 1 shares 95% amino acid sequence identity with mouse Testican 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Testican 1/SPOCK1 Antibody (MAB2327)(2)

Publications using MAB2327

• R Alshargabi, T Sano, A Yamashita, A Takano, T Sanada, M Iwashita, T Shinjo, T Fukuda, T Sanui, S Kishida, F Nishimura SPOCK1 is a novel inducer of epithelial to mesenchymal transition in drug-induced gingival overgrowth Sci Rep, 2020-06-17;10(1):9785. 2020-06-17 [PMID: 32555336] (IHC, Western Blot, Human, Mouse)
• Krishnatry AS, Brazeau DA, Fung HL Broad regulation of matrix and adhesion molecules in THP-1 human macrophages by nitroglycerin. Nitric Oxide, 2009-10-15;22(1):11-7. 2009-10-15 [PMID: 19836459] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SPOCK1
• Uniprot:
  • Q08629()