Fibulin 1 Antibody (CL0337) - BSA Free

Immunohistochemistry-Paraffin: Fibulin 1 Antibody (CL0337) [NBP2-52918] - Staining in human cervix, uterine and liver tissues. Corresponding FBLN1 RNA-seq data are presented for the same tissues.

Western Blot: Fibulin 1 Antibody (CL0337) [NBP2-52918] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-FBLN1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Western Blot: Fibulin 1 Antibody (CL0337) [NBP2-52918] - Lane 1: Marker [kDa] Lane 2: Human plasma

Immunohistochemistry-Paraffin: Fibulin 1 Antibody (CL0337) [NBP2-52918] - Staining of human uterine cervix shows strong positivity in the extracellular matrix.

Immunohistochemistry-Paraffin: Fibulin 1 Antibody (CL0337) [NBP2-52918] - Staining of human prostate shows moderate positivity in the extracellular matrix.

Immunohistochemistry-Paraffin: Fibulin 1 Antibody (CL0337) [NBP2-52918] - Staining of human breast cancer shows moderate positivity in the extracellular matrix of tumor stroma.

Immunohistochemistry-Paraffin: Fibulin 1 Antibody (CL0337) [NBP2-52918] - Staining of human liver shows no positivity as expected.

Staining of MCF7 cells using the Anti-FBLN1 monoclonal antibody, showing no specific staining of vesicles. MCF7 cells serves as a negative control based on RNA-seq values. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Staining of RT-4 cells using the Anti-FBLN1 monoclonal antibody, showing specific staining in vesicles in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clone: CL0337
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Fibulin 1 Antibody (CL0337) - BSA Free Summary

• Description: Novus Biologicals Mouse Fibulin 1 Antibody (CL0337) - BSA Free (NBP2-52918) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Fibulin 1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P23142, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (92%), Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FBLN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Fibulin 1 Recombinant Protein Antigen (NBP2-52918PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Fibulin 1 Antibody (CL0337) - BSA Free

• FBLN
• FBLN1
• FIBL1
• FIBL-1
• Fibulin 1
• fibulin-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibulin 1 Antibody (NBP2-52918)(1)

Publication using NBP2-52918

• Peng L, Jin X, He Q et al. Remodelling landscape of tissue-engineered bladder with porcine small intestine submucosa using single-cell RNA sequencing Cell proliferation 2022-09-30 [PMID: 36177893] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: FBLN1