Fibronectin Antibody [Unconjugated]


Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Rat Fibronectin Antigen Affinity-purified Polyclonal more
Fibronectin was detected in immersion fixed A549 human lung carcinoma cell line untreated (top panel) or treated with 10 ng/mL TGF-beta (Catalog # 240-B, lower panel) for 48 hours using Sheep Anti-Human Fibronectin more
Fibronectin was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) at 10 µg/mL overnight at 4 °C. more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Fibronectin at approximately 230 kDa (as indicated) using 10 µg/mL of more

Product Details

Reactivity Hu, RtSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF

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Fibronectin Antibody [Unconjugated] Summary

Human plasma-derived Fibronectin
Detects human Fibronectin in direct ELISAs. Detects human and rat Fibronectin in Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
AF1918 in the following applications:

Read Publications using
AF1918 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fibronectin Antibody [Unconjugated]

  • CIG
  • ED-B
  • fibronectin 1
  • Fibronectin
  • FINC
  • FN
  • FN1
  • FNZ
  • GFND
  • GFND2
  • LETS
  • MSF


Plasma Fibronectin is a secreted dimeric protein. It is involved in cell adhesion, migration, wound healing, and other cellular processes. Human Fibronectin shares 92% and 90% amino acid sequence homology with bovine and mouse Fibronectin, respectively.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fibronectin Antibody (AF1918)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ELISA Capture, ICC, ICC/IF, IHC, IHC-P, Western Blot.

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ELISA Capture
Western Blot
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Showing Publications 1 - 10 of 10.
Publications using AF1918 Applications Species
D Michalski, W Reimann, E Spielvogel, B Mages, B Biedermann, H Barthel, B Nitzsche, S Schob, W Härtig Regionally Altered Immunosignals of Surfactant Protein-G, Vascular and Non-Vascular Elements of the Neurovascular Unit after Experimental Focal Cerebral Ischemia in Mice, Rats, and Sheep International Journal of Molecular Sciences, 2022;23(11):. 2022 [PMID: 35682557] (IHC, Mouse) IHC Mouse
J Xie, M Sun, D Zhang, C Chen, S Lin, G Zhang Fibronectin enhances tumor metastasis through B7-H3 in clear cell renal cell carcinoma FEBS Open Bio, 2021;0(0):. 2021 [PMID: 34431237] (ELISA Capture, Human) ELISA Capture Human
A Acheva, S Haghdoost, A Sollazzo, V Launonen, M Kämäräinen Presence of Stromal Cells Enhances Epithelial-to-Mesenchymal Transition (EMT) Induction in Lung Bronchial Epithelium after Protracted Exposure to Oxidative Stress of Gamma Radiation Oxid Med Cell Longev, 2019;2019(0):4120379. 2019 [PMID: 31583039] (Western Blot, Human) Western Blot Human
T van Gronin, N Akogul, EM Westerhout, A Chan, NE Hasselt, DA Zwijnenbur, M Broekmans, P Stroeken, F Haneveld, GKJ Hooijer, CD Savci-Heij, A Lakeman, R Volckmann, P van Sluis, LJ Valentijn, J Koster, R Versteeg, J van Nes A NOTCH feed-forward loop drives reprogramming from adrenergic to mesenchymal state in neuroblastoma Nat Commun, 2019;10(1):1530. 2019 [PMID: 30948783] (Western Blot, Human) Western Blot Human
AL Jolly, S Rau, AK Chadha, EA Abdulrahee, D Dean Stromal Fibroblasts Drive Host Inflammatory Responses That Are Dependent on Chlamydia trachomatis Strain Type and Likely Influence Disease Outcomes MBio, 2019;10(2):. 2019 [PMID: 30890604] (ICC/IF, Human) ICC/IF Human
Sandeep Kumar Vishwakarm, Avinash Bardia, Chandrakala Lakkiredd, Syed Ameer Basha Paspala, Aleem Ahmed Khan Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury. Frontiers in Bioengineering and Biotechnology, 2018;0(0):2296-4185. 2018 [PMID: 30443545] (IHC-P, Human) IHC-P Human
T van Gronin, J Koster, LJ Valentijn, DA Zwijnenbur, N Akogul, NE Hasselt, M Broekmans, F Haneveld, NE Nowakowska, J Bras, CJM van Noesel, A Jongejan, AH van Kampen, L Koster, F Baas, L van Dijk-K, M Huizer-Smi, MC Lecca, A Chan, A Lakeman, P Molenaar, R Volckmann, EM Westerhout, M Hamdi, PG van Sluis, ME Ebus, JJ Molenaar, GA Tytgat, BA Westerman, J van Nes, R Versteeg Neuroblastoma is composed of two super-enhancer-associated differentiation states Nat. Genet., 2017;0(0):. 2017 [PMID: 28650485] (Human) Human
PDGFRbeta expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation. J. Cell. Sci., 2012;125(0):2276-87. 2012 [PMID: 22344267] (Western Blot, Human) Western Blot Human
Sabatte J, Faigle W, Ceballos A, Morelle W, Rodrígues CR, Lenicov FR, Thepaut M, Fieschi F, Malchiodi E, Fernandez M, Arenzana-Seisdedos F, Lortat-Jacob H, Michalski JC, Geffner J, Amigorena S Semen clusterin is a novel DC-SIGN ligand. J. Immunol., 2011;187(10):5299-309. 2011 [PMID: 22013110] (Western Blot, Human) Western Blot Human
Massam-Wu T, Chiu M, Choudhury R Assembly of fibrillin microfibrils governs extracellular deposition of latent TGF beta. J. Cell. Sci., 2010;123(0):3006-18. 2010 [PMID: 20699357] (ICC, Human) ICC Human

Review for Fibronectin Antibody (AF1918) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1918:
Filter by Applications
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Images Ratings Applications Species Date Details
reviewed by:
Anonymous -
IF Human 01/05/2015


Sample TestedSee PMID 20699357

Product General Protocols

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By Jamshed Arslan Pharm.D. Nickel (Ni) is a naturally abundant metallic element. It is a major component of stainless steel, coins, and many other items of daily use. Disturbingly, Ni exposure is associated with can...  Read full blog post.

Bad news for stomach cancer: BAMBI protein inhibits gastric carcinoma via TGF-beta/epithelial-mesenchymal transition signaling
By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric ...  Read full blog post.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Fibronectin: Organizing Cell Activity across the ECM
Fibronectin is a glycoprotein found in the extracellular matrix (ECM) that binds to integrins and other components of the ECM such as collagen and fibrin. Under normal physiological conditions, fibronectin is an important factor in cell adhesion, grow...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

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Recent Reviews


Anonymous -
Application: IF
Species: Human


Gene Symbol FN1