Fibronectin Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Rat Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Fibronectin at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Fibronectin was detected in immersion fixed A549 human lung carcinoma cell line untreated (top panel) or treated with 10 ng/mL TGF-beta (Catalog # 240-B, lower panel) for 48 hours using Sheep Anti-Human Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Fibronectin was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Fibronectin at approximately 230 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Rat Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016).The 12-230kDa separation system and EZ Standard Pack 5 are recommended for detecting human Fibronectin using Simple WesternTM

• Reactivity: Hu, Rt
• Applications: WB, Simple Western, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Fibronectin Antibody [Unconjugated] Summary

• Immunogen: Human plasma-derived Fibronectin
• Specificity: Detects human Fibronectin in direct ELISAs. Detects human and rat Fibronectin in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: FN1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fibronectin Antibody [Unconjugated]

• CIG
• ED-B
• fibronectin 1
• Fibronectin
• FINC
• FN
• FN1
• FNZ
• GFND
• GFND2
• LETS
• MSF
• SMDCF

Background

Plasma Fibronectin is a secreted dimeric protein. It is involved in cell adhesion, migration, wound healing, and other cellular processes. Human Fibronectin shares 92% and 90% amino acid sequence homology with bovine and mouse Fibronectin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibronectin Antibody (AF1918)(10)

Publications using AF1918

• D Michalski, W Reimann, E Spielvogel, B Mages, B Biedermann, H Barthel, B Nitzsche, S Schob, W Härtig Regionally Altered Immunosignals of Surfactant Protein-G, Vascular and Non-Vascular Elements of the Neurovascular Unit after Experimental Focal Cerebral Ischemia in Mice, Rats, and Sheep International Journal of Molecular Sciences, 2022;23(11):. 2022 [PMID: 35682557] (IHC, Mouse)
• J Xie, M Sun, D Zhang, C Chen, S Lin, G Zhang Fibronectin enhances tumor metastasis through B7-H3 in clear cell renal cell carcinoma FEBS Open Bio, 2021;0(0):. 2021 [PMID: 34431237] (ELISA Capture, Human)
• A Acheva, S Haghdoost, A Sollazzo, V Launonen, M Kämäräinen Presence of Stromal Cells Enhances Epithelial-to-Mesenchymal Transition (EMT) Induction in Lung Bronchial Epithelium after Protracted Exposure to Oxidative Stress of Gamma Radiation Oxid Med Cell Longev, 2019;2019(0):4120379. 2019 [PMID: 31583039] (Western Blot, Human)
• T van Gronin, N Akogul, EM Westerhout, A Chan, NE Hasselt, DA Zwijnenbur, M Broekmans, P Stroeken, F Haneveld, GKJ Hooijer, CD Savci-Heij, A Lakeman, R Volckmann, P van Sluis, LJ Valentijn, J Koster, R Versteeg, J van Nes A NOTCH feed-forward loop drives reprogramming from adrenergic to mesenchymal state in neuroblastoma Nat Commun, 2019;10(1):1530. 2019 [PMID: 30948783] (Western Blot, Human)
• AL Jolly, S Rau, AK Chadha, EA Abdulrahee, D Dean Stromal Fibroblasts Drive Host Inflammatory Responses That Are Dependent on Chlamydia trachomatis Strain Type and Likely Influence Disease Outcomes MBio, 2019;10(2):. 2019 [PMID: 30890604] (ICC/IF, Human)
• Sandeep Kumar Vishwakarm, Avinash Bardia, Chandrakala Lakkiredd, Syed Ameer Basha Paspala, Aleem Ahmed Khan Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury. Frontiers in Bioengineering and Biotechnology, 2018;0(0):2296-4185. 2018 [PMID: 30443545] (IHC-P, Human)
• T van Gronin, J Koster, LJ Valentijn, DA Zwijnenbur, N Akogul, NE Hasselt, M Broekmans, F Haneveld, NE Nowakowska, J Bras, CJM van Noesel, A Jongejan, AH van Kampen, L Koster, F Baas, L van Dijk-K, M Huizer-Smi, MC Lecca, A Chan, A Lakeman, P Molenaar, R Volckmann, EM Westerhout, M Hamdi, PG van Sluis, ME Ebus, JJ Molenaar, GA Tytgat, BA Westerman, J van Nes, R Versteeg Neuroblastoma is composed of two super-enhancer-associated differentiation states Nat. Genet., 2017;0(0):. 2017 [PMID: 28650485] (Human)
• PDGFRbeta expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation. J. Cell. Sci., 2012;125(0):2276-87. 2012 [PMID: 22344267] (Western Blot, Human)
• Sabatte J, Faigle W, Ceballos A, Morelle W, Rodrígues CR, Lenicov FR, Thepaut M, Fieschi F, Malchiodi E, Fernandez M, Arenzana-Seisdedos F, Lortat-Jacob H, Michalski JC, Geffner J, Amigorena S Semen clusterin is a novel DC-SIGN ligand. J. Immunol., 2011;187(10):5299-309. 2011 [PMID: 22013110] (Western Blot, Human)
• Massam-Wu T, Chiu M, Choudhury R Assembly of fibrillin microfibrils governs extracellular deposition of latent TGF beta. J. Cell. Sci., 2010;123(0):3006-18. 2010 [PMID: 20699357] (ICC, Human)

Review for Fibronectin Antibody (AF1918) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous - IF Human 01/05/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 20699357
• Species: Human

Bioinformatics

• Gene Symbol: FN1