Fibrinogen gamma chain Antibody - BSA Free

Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436] - Mouse plasma and rat plasma (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Fibrinogen gamma chain Antibody [NBP1-32436] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fibrinogen gamma protein stained by Fibrinogen gamma antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: Fibrinogen gamma chain Antibody [NBP1-32436] - Breast cancer. Fibrinogen gamma antibody dilution: 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody diluted at 1:500.

Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436] - Human plasma and HepG2 conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunoprecipitation: Fibrinogen gamma chain Antibody [NBP1-32436] - Fibrinogen gamma protein from HepG2 whole cell extracts using 5 ug of Fibrinogen gamma antibody. Western blot analysis was performed using Fibrinogen gamma antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Immunocytochemistry/ Immunofluorescence: Fibrinogen gamma chain Antibody [NBP1-32436] - Fibrinogen gamma chain antibody detects Fibrinogen gamma chain protein at cytoplasm by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Fibrinogen gamma chain stained by Fibrinogen gamma chain antibody (NBP1-32436) diluted at 1:500.Blue: Fluoroshield with DAPI .Scale bar= 10um.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Fibrinogen gamma chain Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Fibrinogen gamma chain. The exact sequence is proprietary.
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FGG
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:20000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (84%)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Fibrinogen gamma chain Antibody - BSA Free

• fibrinogen gamma chain
• fibrinogen, gamma polypeptide

Background

The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: FGG