Fibrinogen gamma chain Antibody

Images

 
Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Fibrinogen gamma chain Antibody [NBP1-32436] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fibrinogen gamma protein stained by Fibrinogen gamma antibody ...read more
Immunohistochemistry-Paraffin: Fibrinogen gamma chain Antibody [NBP1-32436] - Paraffin-embedded Breast Carcinoma; antibody dilution: 1:250.
Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436] - 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436] - Human plasma and HepG2 conditioned medium were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody diluted at 1:10000.
Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody diluted at 1:500.
Immunoprecipitation: Fibrinogen gamma chain Antibody [NBP1-32436] - Immunoprecipitation of Fibrinogen gamma protein from HepG2 whole cell extracts using 5 ug of Fibrinogen gamma antibody. Western blot analysis was ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Fibrinogen gamma chain Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Fibrinogen gamma. The exact sequence is proprietary.
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FGG
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Porcine (84%), Bovine (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Fibrinogen gamma chain Antibody

  • fibrinogen gamma chain
  • fibrinogen, gamma polypeptide

Background

The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Blogs on Fibrinogen gamma chain.

CD11b Expression, Leukocyte Adhesion and the Innate Immune System
CD11b is an integrin family member which pairs with CD18 to form the CR3 heterodimer. CD11b is expressed on the surface of many leukocytes including monocytes, neutrophils, natural killer cells, granulocytes and macrophages, as well as on 8% of spleen...  Read full blog post.

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Bioinformatics

Gene Symbol FGG